We herein describe a procedure for manufacturing the human serum based control specific for lipid assay, applying the precipitation method by Burstein et al. The control serum is prepared by reconstituting a lyophilized LDL and VLDL depleted serum with distilled water and adding a liquefied LDL and VLDL components to it when in use. The advantageous point this procedure is that the concentration of each lipid component can be changed according to the requirement. The control serum can equally be compared with fresh human serum in its transparency, electrophoretic characteristics and recovery of lipids by ultracentrifugal analysis. Furthermore, the enzymatic analyses of triglycerides, total cholesterol and phospholipids in the control serum the similar reaction kinetics as those of fresh human serum.
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