Preparation of a monoclonal antibody against notoginsenoside R1, a distinctive saponin from panax notoginseng, and its application to indirect competitive elisa

Supattra Limsuwanchote, Juraithip Wungsintaweekul, Gorawit Yusakul, Jing Yan Han, Kaori Tabata, Hiroyuki Tanaka, Yukihiro Shoyama, Satoshi Morimoto

研究成果: ジャーナルへの寄稿記事

8 引用 (Scopus)

抄録

We have prepared a monoclonal antibody against notoginsenoside R1, a primary active constituent of Sanqi ginseng (roots of Panax notoginseng). The monoclonal antibody was raised by immunizing BALB/c male mice with notoginsenoside R1-bovine albumin conjugates following cell fusion via electroporation. This method has been shown to be very effective for producing hybridomas with excellent antibody prevalence following cell fusion of their splenocytes with cells of the myeloma cell line SP2/0. Of all the hybridomas secreting a monoclonal antibody against notoginsenoside R1, only the 1A1P cell line produces a highly specific antibody to this compound. Surprisingly, the cross-reactivity of this monoclonal antibody for ginsenoside Rg1 and ginsenoside Re, derivatives of protopanaxatriol, was below 1.02% in a competitive immunoassay. Based on this characteristic of the monoclonal antibody, an indirect competitive ELISA (range of measurement 0.56-9 μg/mL) was established and applied as a quality control method. In conclusion, the developed immunoassay was easy to handle and reliable for the analysis of notoginsenoside R1 in Sanqi ginseng products without requiring a pretreatment.

元の言語英語
ページ(範囲)337-342
ページ数6
ジャーナルPlanta medica
80
発行部数4
DOI
出版物ステータス出版済み - 1 1 2014

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Panax notoginseng
Saponins
Monoclonal Antibodies
Panax
Cell Fusion
Hybridomas
Immunoassay
Fusion reactions
Cells
Cell Line
Electroporation
Antibodies
Quality Control
Quality control
Albumins
Enzyme-Linked Immunosorbent Assay
notoginsenoside R1
Derivatives

All Science Journal Classification (ASJC) codes

  • Analytical Chemistry
  • Molecular Medicine
  • Pharmacology
  • Pharmaceutical Science
  • Drug Discovery
  • Complementary and alternative medicine
  • Organic Chemistry

これを引用

Preparation of a monoclonal antibody against notoginsenoside R1, a distinctive saponin from panax notoginseng, and its application to indirect competitive elisa. / Limsuwanchote, Supattra; Wungsintaweekul, Juraithip; Yusakul, Gorawit; Han, Jing Yan; Tabata, Kaori; Tanaka, Hiroyuki; Shoyama, Yukihiro; Morimoto, Satoshi.

:: Planta medica, 巻 80, 番号 4, 01.01.2014, p. 337-342.

研究成果: ジャーナルへの寄稿記事

Limsuwanchote, Supattra ; Wungsintaweekul, Juraithip ; Yusakul, Gorawit ; Han, Jing Yan ; Tabata, Kaori ; Tanaka, Hiroyuki ; Shoyama, Yukihiro ; Morimoto, Satoshi. / Preparation of a monoclonal antibody against notoginsenoside R1, a distinctive saponin from panax notoginseng, and its application to indirect competitive elisa. :: Planta medica. 2014 ; 巻 80, 番号 4. pp. 337-342.
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abstract = "We have prepared a monoclonal antibody against notoginsenoside R1, a primary active constituent of Sanqi ginseng (roots of Panax notoginseng). The monoclonal antibody was raised by immunizing BALB/c male mice with notoginsenoside R1-bovine albumin conjugates following cell fusion via electroporation. This method has been shown to be very effective for producing hybridomas with excellent antibody prevalence following cell fusion of their splenocytes with cells of the myeloma cell line SP2/0. Of all the hybridomas secreting a monoclonal antibody against notoginsenoside R1, only the 1A1P cell line produces a highly specific antibody to this compound. Surprisingly, the cross-reactivity of this monoclonal antibody for ginsenoside Rg1 and ginsenoside Re, derivatives of protopanaxatriol, was below 1.02{\%} in a competitive immunoassay. Based on this characteristic of the monoclonal antibody, an indirect competitive ELISA (range of measurement 0.56-9 μg/mL) was established and applied as a quality control method. In conclusion, the developed immunoassay was easy to handle and reliable for the analysis of notoginsenoside R1 in Sanqi ginseng products without requiring a pretreatment.",
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