Preparation of anti-fragrant monoclonal antibodies by the rat lymph node method and their characterization using enzyme-linked immunosorbent assay

Shintaro Ohashi, Kazutaka Nagatomo, Koichiro Miyamoto, Kiyoshi Toko, Norio Miura, Kiyoshi Matsumoto

研究成果: Contribution to journalArticle査読

3 被引用数 (Scopus)

抄録

In the food and beverage industries there is a great demand for a sensitive and easy detection system of fragrant compounds in order to ensure product quality control and to avoid contamination Benzaldehyde (BZ) and furfural (FF) are typical fragrant compounds for peach-flavored beverages, and methyl anthranilate (MA) is a typical flavor of grape beverages. For sensitive detection of these flavors by immunoreaction, we prepared monoclonal antibodies against BZ, FF and MA by using the rat lymph node method. The antibody for BZ had high selectivity toward BZ compared with other fragrant compounds and high affinity with an affinity constant of 1.9x10 6M -1. The limit of detection (LOD) of BZ by indirect competitive ELISA (ic-ELISA) was 0.6 ng mL -1. For FF, anti-FF mAb had high selectivity toward FF and high affinity constant of 2.5x10 -1 M -1. The LOD of FF by ic-ELISA was 100 ng mL -1. For MA, anti-MA mAb had high selectivity toward MA and high affinity constant of 1.3x10 5M -1. The LOD of MA by ic-ELISA was 200 ng mL -1. These monoclonal antibodies were suitable for detection of very small amounts of fragrant compounds in rinsed water of beverage industries.

本文言語英語
ページ(範囲)91-96
ページ数6
ジャーナルJournal of the Faculty of Agriculture, Kyushu University
55
1
出版ステータス出版済み - 2 2010

All Science Journal Classification (ASJC) codes

  • バイオテクノロジー
  • 農業および作物学

フィンガープリント

「Preparation of anti-fragrant monoclonal antibodies by the rat lymph node method and their characterization using enzyme-linked immunosorbent assay」の研究トピックを掘り下げます。これらがまとまってユニークなフィンガープリントを構成します。

引用スタイル