Preparation of cell-culturing glass surfaces that release branched polyethyleneimine triggered by thiol-disulfide exchange

Aya Mizutani Akimoto, Tohru Takarada, Mizuo Maeda

研究成果: ジャーナルへの寄稿記事

1 引用 (Scopus)

抄録

To develop a chemical stimulus-responsive substrate for culturing cells, polyethyleneimine (PEI) having a pyridyl disulfide moiety was attached via disulfide linkages to a glass coverslip modified with a silane coupling agent having a thiol group. The surface modification was confirmed by X-ray photoelectron spectroscopy and zeta potential analysis. The obtained surface exhibited sufficiently high cell adhesiveness. Zeta potential measurements revealed that the PEI derivatives were released from the surface through thiol-disulfide exchange when the modified glass coverslip was immersed in a neutral pH buffer containing cysteine. The cell viability assay demonstrated that this chemical stimulus was substantially nontoxic to 293T cells. Because PEI is a widely used transfection reagent, this functional glass coverslip would be potentially useful as an experimental platform for reverse transfection.

元の言語英語
ページ(範囲)360-365
ページ数6
ジャーナルColloids and Surfaces B: Biointerfaces
103
DOI
出版物ステータス出版済み - 3 1 2013
外部発表Yes

Fingerprint

cell culturing
Polyethyleneimine
disulfides
Sulfhydryl Compounds
thiols
Disulfides
Glass
Zeta potential
stimuli
preparation
Transfection
glass
Silanes
Adhesiveness
Photoelectron Spectroscopy
HEK293 Cells
Coupling agents
cysteine
cells
viability

All Science Journal Classification (ASJC) codes

  • Biotechnology
  • Surfaces and Interfaces
  • Physical and Theoretical Chemistry
  • Colloid and Surface Chemistry

これを引用

Preparation of cell-culturing glass surfaces that release branched polyethyleneimine triggered by thiol-disulfide exchange. / Akimoto, Aya Mizutani; Takarada, Tohru; Maeda, Mizuo.

:: Colloids and Surfaces B: Biointerfaces, 巻 103, 01.03.2013, p. 360-365.

研究成果: ジャーナルへの寄稿記事

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