Preparation of homogenous oligosaccharide chains from glycosphingolipids

Yu Teh Li, Chau Wen Chou, Su Chen Li, Utaro Kobayashi, Yo Hey Ishibashi, Makoto Ito

研究成果: Contribution to journalArticle査読

18 被引用数 (Scopus)

抄録

After the discovery of glycosphingolipid (GSL) glycan detaching enzymes, Rhodococcal endoglycoceramidase (EGCase) and leech ceramide glycanase (CGase), the method for enzymatically releasing glycans from GSLs has become the method of choice for preparing intact ceramide-free oligosaccharide chains from GSLs. This paper describes (1) the preparation of the intact oligosaccharides from GM1 (II3NeuAcGgOse4Cer) and GbOse4Cer as examples to show the use of CGase to prepare intact glycan chains from GSLs, and (2) the specificity and detergent requirements of Rhodococcal EGCases for the release of glycan chains from different GSLs.

本文言語英語
ページ(範囲)929-933
ページ数5
ジャーナルGlycoconjugate Journal
26
8
DOI
出版ステータス出版済み - 11 2009

All Science Journal Classification (ASJC) codes

  • Biochemistry
  • Molecular Biology
  • Cell Biology

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