Preparation of homogenous oligosaccharide chains from glycosphingolipids

Yu Teh Li, Chau Wen Chou, Su Chen Li, Utaro Kobayashi, Yohei Ishibashi, Makoto Ito

研究成果: ジャーナルへの寄稿記事

17 引用 (Scopus)

抄録

After the discovery of glycosphingolipid (GSL) glycan detaching enzymes, Rhodococcal endoglycoceramidase (EGCase) and leech ceramide glycanase (CGase), the method for enzymatically releasing glycans from GSLs has become the method of choice for preparing intact ceramide-free oligosaccharide chains from GSLs. This paper describes (1) the preparation of the intact oligosaccharides from GM1 (II3NeuAcGgOse4Cer) and GbOse4Cer as examples to show the use of CGase to prepare intact glycan chains from GSLs, and (2) the specificity and detergent requirements of Rhodococcal EGCases for the release of glycan chains from different GSLs.

元の言語英語
ページ(範囲)929-933
ページ数5
ジャーナルGlycoconjugate Journal
26
発行部数8
DOI
出版物ステータス出版済み - 11 1 2009

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Glycosphingolipids
Oligosaccharides
Polysaccharides
endoglycoceramidase
Leeches
Ceramides
Detergents
Enzymes
ceramide glycanase

All Science Journal Classification (ASJC) codes

  • Biochemistry
  • Molecular Biology
  • Cell Biology

これを引用

Preparation of homogenous oligosaccharide chains from glycosphingolipids. / Li, Yu Teh; Chou, Chau Wen; Li, Su Chen; Kobayashi, Utaro; Ishibashi, Yohei; Ito, Makoto.

:: Glycoconjugate Journal, 巻 26, 番号 8, 01.11.2009, p. 929-933.

研究成果: ジャーナルへの寄稿記事

Li, Yu Teh ; Chou, Chau Wen ; Li, Su Chen ; Kobayashi, Utaro ; Ishibashi, Yohei ; Ito, Makoto. / Preparation of homogenous oligosaccharide chains from glycosphingolipids. :: Glycoconjugate Journal. 2009 ; 巻 26, 番号 8. pp. 929-933.
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