Preparation of surfactant-coated lipases utilizing the molecular imprinting technique

研究成果: Contribution to journalArticle査読

13 被引用数 (Scopus)

抄録

Modulation of the enantioselectivity of two lipases exhibiting different specificities in the esterification of 2-octanol with lauric acid was investigated by utilizing surfactant-coating and molecular imprinting techniques. The enantioselectivity of the surfactant-coated lipase from Pseudomonas cepacia (PS) was enhanced in the presence of (R)-2-octanol as a print molecule, whereas the enantioselectivity of the lipase from Candida cylindracea (AY) was hardly changed by the imprinting technique. In the case of lipase AY, however, the selectivity of the native enzyme toward the (R)-isomer was changed to a preference for the (S)-isomer as a result of being coating with surfactant molecules. The molecular imprinting effect was prominent in the case of lipase PS, its enantioselectivity in the formation of 2-octanoate in isooctane being enhanced around two-fold compared with that of the native enzyme.

本文言語英語
ページ(範囲)237-239
ページ数3
ジャーナルJournal of Fermentation and Bioengineering
85
2
DOI
出版ステータス出版済み - 1998

All Science Journal Classification (ASJC) codes

  • バイオテクノロジー
  • 応用微生物学とバイオテクノロジー

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