Preparation of thermoresponsive anionic copolymer brush surfaces for separating basic biomolecules

Kenichi Nagase, Jun Kobayashi, Akihiko Kikuchi, Yoshikatsu Akiyama, Hideko Kanazawa, Masahiko Annaka, Teruo Okano

研究成果: ジャーナルへの寄稿記事

31 引用 (Scopus)

抄録

Poly(N-isopropylacrylamide-co-acrylic acid-co-N-tert-butylacrylamide) (poly(IPAAm-co-AAc-co-tBAAm) brush grafted silica beads were prepared through a surface-initiated atom transfer radical polymerization (ATRP) with CuCl/CuCl2/Me6TREN catalytic system in 2-propanol at 25 °C for 4 h. The prepared beads were characterized by chromatographic analysis. Basic analytes, catecholamine derivatives, and angiotensin peptides could be separated by a short column length containing the beads because of its high densely grafted copolymer structure. Chromatograms for catecholamine derivatives were obtained with high resolution peaks due to their electrostatic and hydrophobic interactions to the densely grafted anionic copolymers on the beads. Effective separation of angiotensin peptides was performed near the lower critical solution temperature of copolymers, because the total electrostatic and hydrophobic interactions between the copolymer and the analytes become strong at the temperature. These results indicated that the copolymer brush grafted surfaces prepared by ATRP was an effective tool for separating basic biomolecules by modulating the electrostatic and hydrophobic interactions.

元の言語英語
ページ(範囲)215-223
ページ数9
ジャーナルBiomacromolecules
11
発行部数1
DOI
出版物ステータス出版済み - 1 11 2010

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Biomolecules
Brushes
Copolymers
Electrostatics
Atom transfer radical polymerization
Angiotensins
Peptides
Catecholamines
Chromatographic analysis
Derivatives
2-Propanol
Propanol
Silicon Dioxide
Acrylics
Silica
Temperature
Acids

All Science Journal Classification (ASJC) codes

  • Bioengineering
  • Biomaterials
  • Polymers and Plastics
  • Materials Chemistry

これを引用

Nagase, K., Kobayashi, J., Kikuchi, A., Akiyama, Y., Kanazawa, H., Annaka, M., & Okano, T. (2010). Preparation of thermoresponsive anionic copolymer brush surfaces for separating basic biomolecules. Biomacromolecules, 11(1), 215-223. https://doi.org/10.1021/bm9010744

Preparation of thermoresponsive anionic copolymer brush surfaces for separating basic biomolecules. / Nagase, Kenichi; Kobayashi, Jun; Kikuchi, Akihiko; Akiyama, Yoshikatsu; Kanazawa, Hideko; Annaka, Masahiko; Okano, Teruo.

:: Biomacromolecules, 巻 11, 番号 1, 11.01.2010, p. 215-223.

研究成果: ジャーナルへの寄稿記事

Nagase, K, Kobayashi, J, Kikuchi, A, Akiyama, Y, Kanazawa, H, Annaka, M & Okano, T 2010, 'Preparation of thermoresponsive anionic copolymer brush surfaces for separating basic biomolecules', Biomacromolecules, 巻. 11, 番号 1, pp. 215-223. https://doi.org/10.1021/bm9010744
Nagase, Kenichi ; Kobayashi, Jun ; Kikuchi, Akihiko ; Akiyama, Yoshikatsu ; Kanazawa, Hideko ; Annaka, Masahiko ; Okano, Teruo. / Preparation of thermoresponsive anionic copolymer brush surfaces for separating basic biomolecules. :: Biomacromolecules. 2010 ; 巻 11, 番号 1. pp. 215-223.
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