Prevention of nuclear mixing of fused cells interconnected through a microslit using rock inhibitor y-27632 for direct cytoplasmic transfer between live single cells

K. I. Wada, K. Hosokawa, Y. Ito, M. Maeda

研究成果: Chapter in Book/Report/Conference proceedingConference contribution

抄録

We previously reported a cell fusion method using a microfluidic device, in which paired single cells were fused through a microslit [1]. Although our approach realized direct cytoplasmic transfer between live single cells and thus is a promising method to induce cellular reprogramming or transdifferentiation, prevention of nuclear mixing with the microslit was unsatisfactory (∼15%). We herein report that the nuclear mixing was almost perfectly prevented (∼95%) by addition of ROCK inhibitor Y-27632 in NIH3T3 fibroblast. Furthermore, Y-27632 raised the probability of cell fusion through the microslit. As a result, we obtained 16.5-fold increase in the number of nuclear-separated fused cell pairs.

本文言語英語
ホスト出版物のタイトルMicroTAS 2015 - 19th International Conference on Miniaturized Systems for Chemistry and Life Sciences
出版社Chemical and Biological Microsystems Society
ページ792-794
ページ数3
ISBN(電子版)9780979806483
出版ステータス出版済み - 2015
外部発表はい
イベント19th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2015 - Gyeongju, 大韓民国
継続期間: 10 25 201510 29 2015

出版物シリーズ

名前MicroTAS 2015 - 19th International Conference on Miniaturized Systems for Chemistry and Life Sciences

その他

その他19th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2015
国/地域大韓民国
CityGyeongju
Period10/25/1510/29/15

All Science Journal Classification (ASJC) codes

  • 制御およびシステム工学

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