Primary rat hepatocytes form spheroids on hepatocyte growth factor/heparin-immobilized collagen film and maintain high albumin production

Hiroyuki Ijima, Takafumi Kubo, Yung Te Hou

研究成果: ジャーナルへの寄稿学術誌査読

16 被引用数 (Scopus)

抄録

We developed hepatocyte growth factor (HGF)/heparin-immobilized collagen film by adding HGF solution to collagen film, which immobilized heparin by chemical cross-linking. Primary rat hepatocytes maintained neither their specific morphology nor albumin synthesis on collagen film that directly immobilized HGF by cross-linking. By using HGF-supplemented medium, hepatocytes on collagen film formed typical monolayers and maintained albumin synthesis. Hepatocytes on HGF/heparin-immobilized collagen film formed spheroids after 3 days. They maintained albumin production during the culture period, the same as in HGF-supplemented medium. However, spheroids were not formed although the adhesion of hepatocytes was disturbed on heparin-immobilized collagen film, and albumin synthesis rapidly decreased during 5 days of culture. Because the mitochondrial activities of hepatocytes were not increased in all conditions, it was supposed that the cell proliferation did not occur. Therefore, it was shown that HGF/heparin-immobilized collagen film was effective for promotion of spheroid formation of hepatocytes and expression of their albumin production. Crown

本文言語英語
ページ(範囲)227-233
ページ数7
ジャーナルBiochemical Engineering Journal
46
2
DOI
出版ステータス出版済み - 10月 1 2009

!!!All Science Journal Classification (ASJC) codes

  • バイオテクノロジー
  • 環境工学
  • バイオエンジニアリング
  • 生体医工学

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