Primary structure and transcriptional regulation of rat pepsinogen C gene

Takeshi Ishihara, Y. Ichihara, T. Hayano, I. Katsura, K. Sogawa, Y. Fujii-Kuriyama, K. Takahashi

研究成果: ジャーナルへの寄稿記事

34 引用 (Scopus)

抄録

The entire rat pepsinogen C gene has been isolated from a rat genomic library, using the rat pepsinogen C cDNA as a probe. Southern blot analysis showed that there exists at least two rat pepsinogen C genes. The nucleotide sequences of the coding regions and the 5'- and 3'-flanking regions of one of the rat pepsinogen C genes have been determined. This gene is split into 9 exons interrupted by eight intervening sequences. The 5'-flanking region is similar to that of the human pepsinogen C gene, but only the former had the core sequence of the Sp1 binding site. The amount of transcripts of the rat pepsinogen C genes was found to increase during development, and a similar increase was shown to be induced by injection of hydrocortisone. As a candidate of a factor which regulates the transcription, we found a 25-kDa protein by Southwestern blotting. It binds to a specific site in the 5'-flanking region of the gene only in the presence of Mg2+ ion, and it is present in the nuclear fraction of the gastric mucosa but not of the liver.

元の言語英語
ページ(範囲)10193-10199
ページ数7
ジャーナルJournal of Biological Chemistry
264
発行部数17
出版物ステータス出版済み - 1 1 1989
外部発表Yes

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Pepsinogen C
Rats
Genes
5' Flanking Region
Southwestern Blotting
3' Flanking Region
Genomic Library
Transcription
Southern Blotting
Gastric Mucosa
Liver
Introns
Hydrocortisone
Exons
Nucleotides
Complementary DNA
Binding Sites
Ions
Injections

All Science Journal Classification (ASJC) codes

  • Biochemistry
  • Molecular Biology
  • Cell Biology

これを引用

Ishihara, T., Ichihara, Y., Hayano, T., Katsura, I., Sogawa, K., Fujii-Kuriyama, Y., & Takahashi, K. (1989). Primary structure and transcriptional regulation of rat pepsinogen C gene. Journal of Biological Chemistry, 264(17), 10193-10199.

Primary structure and transcriptional regulation of rat pepsinogen C gene. / Ishihara, Takeshi; Ichihara, Y.; Hayano, T.; Katsura, I.; Sogawa, K.; Fujii-Kuriyama, Y.; Takahashi, K.

:: Journal of Biological Chemistry, 巻 264, 番号 17, 01.01.1989, p. 10193-10199.

研究成果: ジャーナルへの寄稿記事

Ishihara, T, Ichihara, Y, Hayano, T, Katsura, I, Sogawa, K, Fujii-Kuriyama, Y & Takahashi, K 1989, 'Primary structure and transcriptional regulation of rat pepsinogen C gene', Journal of Biological Chemistry, 巻. 264, 番号 17, pp. 10193-10199.
Ishihara T, Ichihara Y, Hayano T, Katsura I, Sogawa K, Fujii-Kuriyama Y その他. Primary structure and transcriptional regulation of rat pepsinogen C gene. Journal of Biological Chemistry. 1989 1 1;264(17):10193-10199.
Ishihara, Takeshi ; Ichihara, Y. ; Hayano, T. ; Katsura, I. ; Sogawa, K. ; Fujii-Kuriyama, Y. ; Takahashi, K. / Primary structure and transcriptional regulation of rat pepsinogen C gene. :: Journal of Biological Chemistry. 1989 ; 巻 264, 番号 17. pp. 10193-10199.
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