Primary structure of an N-linked sugar chain derived from glucoamylase of Rhizopus niveus

Kaoru Takegawa, Naomi Kawasaki, Shojiro Iwahara, Kenji Yamamoto, Tatsurokuro Tochikura, Bunzo Mikami, Yuhei Morita

研究成果: ジャーナルへの寄稿記事

4 引用 (Scopus)

抄録

The primary structure of the N-linked sugar chain of Rhizopus niveus glucoamylase (major component) was investigated. The carbohydrate moiety was released from the polypeptide backbone by Flavobacterium sp. endo-β-N-acetylglucosaminidase digestion. Studies using the method of exoglycosidase digestion of the fluorescent pyridylamino derivative, gel-permeation chromatography on Bio-Gel P-4 and 400-MHz 1H-NMR spectroscopy revealed that the most abundant structure is (Man)8-GlcNAc-ol.

元の言語英語
ページ(範囲)98-100
ページ数3
ジャーナルBiochimica et Biophysica Acta - General Subjects
990
発行部数1
DOI
出版物ステータス出版済み - 1 1 1989
外部発表Yes

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Glucan 1,4-alpha-Glucosidase
Rhizopus
Acetylglucosaminidase
Glycoside Hydrolases
Gel permeation chromatography
Sugars
Nuclear magnetic resonance spectroscopy
Digestion
Carbohydrates
Flavobacterium
Derivatives
Peptides
Gel Chromatography
Magnetic Resonance Spectroscopy
Bio-Gel P-4
endo-alpha-sialidase
N-acetylchitooctaose
Proton Magnetic Resonance Spectroscopy

All Science Journal Classification (ASJC) codes

  • Biophysics
  • Biochemistry
  • Molecular Biology

これを引用

Primary structure of an N-linked sugar chain derived from glucoamylase of Rhizopus niveus. / Takegawa, Kaoru; Kawasaki, Naomi; Iwahara, Shojiro; Yamamoto, Kenji; Tochikura, Tatsurokuro; Mikami, Bunzo; Morita, Yuhei.

:: Biochimica et Biophysica Acta - General Subjects, 巻 990, 番号 1, 01.01.1989, p. 98-100.

研究成果: ジャーナルへの寄稿記事

Takegawa, Kaoru ; Kawasaki, Naomi ; Iwahara, Shojiro ; Yamamoto, Kenji ; Tochikura, Tatsurokuro ; Mikami, Bunzo ; Morita, Yuhei. / Primary structure of an N-linked sugar chain derived from glucoamylase of Rhizopus niveus. :: Biochimica et Biophysica Acta - General Subjects. 1989 ; 巻 990, 番号 1. pp. 98-100.
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AU - Morita, Yuhei

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