Production and Analysis of Hh 10 Monoclonal Antibodies Reactive to Immature Hematopoietic Cells and Their Use for Monitoring Acute Leukemia Cells

Koji Kato, Tomoki Naoe, Reiko Namikawa, Sakae Suzuki, Akikazu Hiraiwa, Kazumasa Yamada, Hiroshi Shiku

研究成果: ジャーナルへの寄稿記事

9 引用 (Scopus)

抄録

A clone of murine monoclonal antibody HH 10 (IgM) was raised by fusing NS-1 myeloma cells and spleen cells of a mouse hyperimmunized with acute promyelocytic leukemia cells. Serological analysis by means of immune adherence assays showed that HH 10 reacts with immature hematopoietic cells including thymocytes and myeloid precursor cells (defined as colony-forming units in culture assays). The antibodies were not reactive to either peripheral blood cells or bone marrow cells obtained from normal individuals. Lymphoid blasts induced with phytohemagglutinin, pokeweed mitogen, or concanavalin A were also non-reactive, and all non-hematopoietic cultured cells examined were also negative. The antibodies were, however, reactive to leukemia cells in 67 cases out of 91 cases (74%) of acute leukemia.

元の言語英語
ページ(範囲)524-531
ページ数8
ジャーナルJapanese Journal of Cancer Research GANN
76
発行部数6
出版物ステータス出版済み - 1 1 1985

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Leukemia
Monoclonal Antibodies
Pokeweed Mitogens
Acute Promyelocytic Leukemia
Antibodies
Phytohemagglutinins
Myeloid Cells
Thymocytes
Concanavalin A
Bone Marrow Cells
Immunoglobulin M
Cultured Cells
Blood Cells
Stem Cells
Spleen
Clone Cells

All Science Journal Classification (ASJC) codes

  • Oncology
  • Cancer Research

これを引用

Production and Analysis of Hh 10 Monoclonal Antibodies Reactive to Immature Hematopoietic Cells and Their Use for Monitoring Acute Leukemia Cells. / Kato, Koji; Naoe, Tomoki; Namikawa, Reiko; Suzuki, Sakae; Hiraiwa, Akikazu; Yamada, Kazumasa; Shiku, Hiroshi.

:: Japanese Journal of Cancer Research GANN, 巻 76, 番号 6, 01.01.1985, p. 524-531.

研究成果: ジャーナルへの寄稿記事

Kato, Koji ; Naoe, Tomoki ; Namikawa, Reiko ; Suzuki, Sakae ; Hiraiwa, Akikazu ; Yamada, Kazumasa ; Shiku, Hiroshi. / Production and Analysis of Hh 10 Monoclonal Antibodies Reactive to Immature Hematopoietic Cells and Their Use for Monitoring Acute Leukemia Cells. :: Japanese Journal of Cancer Research GANN. 1985 ; 巻 76, 番号 6. pp. 524-531.
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abstract = "A clone of murine monoclonal antibody HH 10 (IgM) was raised by fusing NS-1 myeloma cells and spleen cells of a mouse hyperimmunized with acute promyelocytic leukemia cells. Serological analysis by means of immune adherence assays showed that HH 10 reacts with immature hematopoietic cells including thymocytes and myeloid precursor cells (defined as colony-forming units in culture assays). The antibodies were not reactive to either peripheral blood cells or bone marrow cells obtained from normal individuals. Lymphoid blasts induced with phytohemagglutinin, pokeweed mitogen, or concanavalin A were also non-reactive, and all non-hematopoietic cultured cells examined were also negative. The antibodies were, however, reactive to leukemia cells in 67 cases out of 91 cases (74{\%}) of acute leukemia.",
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AU - Kato, Koji

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AU - Suzuki, Sakae

AU - Hiraiwa, Akikazu

AU - Yamada, Kazumasa

AU - Shiku, Hiroshi

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