Production and characterization of N-acyl-D-glutamate amidohydrolase from Pseudomonas sp. strain 5f-1

K. Sakai, K. Oshima, M. Moriguchi

研究成果: Contribution to journalArticle査読

23 被引用数 (Scopus)

抄録

N-Acyl-D-glutamate amidohydrolase from Pseudomonas sp. strain 5f-1 was inducibly produced by D isomers of N-acetylglutamate, glutamate, aspartate, and asparagine. The enzyme has been purified to homogeneity by DEAE-cellulose, (NH4)2SO4 fractionation, and chromatofocusing followed by gel filtration on a Sephadex G-100 column. The enzyme was a monomer with molecular weight of 55,000. The enzyme activity was optimal at pH 6.5 to 7.5 and 45°C. The isoelectric point and the pH stability were 8.8 and 9.0, respectively. N-Formyl, N-acetyl, N-butyryl, N-propionyl, N-chloroacetyl derivatives of D-glutamate and glycyl-D-glutamate were substrates for the enzyme. At pH 6.5 in 100 mM N-2-hydroxyethylpiperazine-N'-2-ethanesulfonic acid (HEPES) buffer at 30°C, a K(m) of 6.67 mM and a V(max) of 662 μmol/min/mg of protein for N-acetyl-D-glutamate were obtained. None of the metal ions stimulated the enzyme activity. Na+, K+, Mg2+, and Ba2+ acted as stabilizers. Hg2+, Cu2+, Zn2+, Fe3+, and EDTA were strongly inhibitory.

本文言語英語
ページ(範囲)2540-2543
ページ数4
ジャーナルApplied and environmental microbiology
57
9
DOI
出版ステータス出版済み - 1991
外部発表はい

All Science Journal Classification (ASJC) codes

  • バイオテクノロジー
  • 食品科学
  • 応用微生物学とバイオテクノロジー
  • 生態学

フィンガープリント

「Production and characterization of N-acyl-D-glutamate amidohydrolase from Pseudomonas sp. strain 5f-1」の研究トピックを掘り下げます。これらがまとまってユニークなフィンガープリントを構成します。

引用スタイル