Production of a monoclonal antibody for C/EBPβ: The subnuclear localization of C/EBPβ in mouse L929 cells

Akihito Harada, Etsuko Okazaki, Seiji Okada, Taro Tachibana, Yasuyuki Ohkawa

研究成果: Contribution to journalArticle査読

1 被引用数 (Scopus)

抄録

The CCAAT/enhancer-binding protein (C/EBP)β belongs to the C/EBP family of proteins that possesses a basic leucine zipper DNA-binding domain. These proteins bind DNA by dimerization and play a role in the transcriptional regulation of various cells. There are six different types of C/EBPs, and some form isoforms through the use of alternative translation initiation sites. The functional analysis of the C/EBP family is therefore difficult to achieve. Here we report on the production of specific monoclonal antibodies against mouse C/EBPβ using a rat medial iliac lymph node method. Immunoblotting using C/EBPβ monoclonal antibodies identified two types of isoforms, while immunostaining revealed a subnuclear localization for C/EBPβ. Use of this antibody should contribute to the further elucidation of the transcriptional regulatory function of C/EBPβ.

本文言語英語
ページ(範囲)34-37
ページ数4
ジャーナルMonoclonal Antibodies in Immunodiagnosis and Immunotherapy
33
1
DOI
出版ステータス出版済み - 2 1 2014

All Science Journal Classification (ASJC) codes

  • Immunology and Allergy
  • Immunology

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