Production of a rat monoclonal antibody against Brg1

Yasuyuki Ohkawa, Akihito Harada, Mako Nakamura, Saori Yoshimura, Taro Tachibana

研究成果: ジャーナルへの寄稿記事

15 引用 (Scopus)

抄録

Brm-related gene-1 (Brg1) is a catalytic subunit of the SWI/SNF chromatin remodeling enzyme complex that has ATPase activity. This complex facilitates chromatin remodeling for gene expression by utilizing energy for ATP hydrolysis. It is well known that the SWI/SNF chromatin remodeling enzyme complex is essential for cell differentiation, cell cycle regulation, and embryogenesis. Here we report the establishment of a hybridoma cell line for producing an antibody against Brg1 subunit by the rat medial iliac lymph node method. Immunoblot analysis showed that our antibody can specifically recognize Brg1. It was revealed by immunocytochemistry that Brg1 is located in euchromatin of C2C12 myoblast nuclei. These data suggested this antibody is useful for analyzing molecular function of Brg1 protein in cells.

元の言語英語
ページ(範囲)463-466
ページ数4
ジャーナルHybridoma
28
発行部数6
DOI
出版物ステータス出版済み - 12 1 2009

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Chromatin Assembly and Disassembly
Monoclonal Antibodies
Genes
Antibodies
Euchromatin
Myoblasts
Hybridomas
Enzymes
Embryonic Development
Adenosine Triphosphatases
Cell Differentiation
Catalytic Domain
Cell Cycle
Hydrolysis
Adenosine Triphosphate
Lymph Nodes
Immunohistochemistry
Gene Expression
Cell Line
Proteins

All Science Journal Classification (ASJC) codes

  • Immunology
  • Immunology and Allergy

これを引用

Production of a rat monoclonal antibody against Brg1. / Ohkawa, Yasuyuki; Harada, Akihito; Nakamura, Mako; Yoshimura, Saori; Tachibana, Taro.

:: Hybridoma, 巻 28, 番号 6, 01.12.2009, p. 463-466.

研究成果: ジャーナルへの寄稿記事

Ohkawa, Yasuyuki ; Harada, Akihito ; Nakamura, Mako ; Yoshimura, Saori ; Tachibana, Taro. / Production of a rat monoclonal antibody against Brg1. :: Hybridoma. 2009 ; 巻 28, 番号 6. pp. 463-466.
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