Production of a tributyltin-binding protein 2 knockout mutant strain of Japanese medaka, Oryzias latipes

Yoko Kato-Unoki, Yuki Takai, Yosuke Nagano, Satoshi Matsunaga, Shintaro Enoki, Takumi Takamura, Sangwan Kim, Masato Kinoshita, Takeshi Kitano, Yohei Shimasaki, Yuji Oshima

研究成果: Contribution to journalArticle査読

抄録

Tributyltin-binding proteins (TBT-bps), members of the lipocalin family, bind TBT in fish blood and are presumed to contribute to detoxification of TBT. Recent studies have shown that many fish species have TBT-bp genes, and that these genes are induced by stresses such as exposure to chemicals or fish pathogenic bacteria. However, the function of TBT-bps, and the mechanisms of their induction and detoxification activity are still unclear. Here, towards elucidating the functions of TBT-bp2, we produced a TBT-bp2 knockout (TBT-bp2−/−) strain of Japanese medaka, Oryzias latipes, by using the CRISPR/Cas9 system. Gene expression of the mutated TBT-bp2 was reduced, and the cDNA sequencing and predicted protein structure suggested possible loss of function. However, the fish could be grown under normal conditions. Exposure of the TBT-bp2−/− strain of medaka to various stresses in future experiments is expected to contribute to our understanding of this novel detoxification system in aquatic organisms.

本文言語英語
論文番号111601
ジャーナルMarine Pollution Bulletin
160
DOI
出版ステータス出版済み - 11 2020

All Science Journal Classification (ASJC) codes

  • 海洋学
  • 水圏科学
  • 汚染

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