Production of heterologous proteins using the fission-yeast (Schizosaccharomyces pombe) expression system

Kaoru Takegawa, Hideki Tohda, Mayumi Sasaki, Alimjan Idiris, Takao Ohashi, Hiroyuki Mukaiyama, Yuko Giga-Hama, Hiromichi Kumagai

研究成果: Contribution to journalShort survey査読

50 被引用数 (Scopus)

抄録

The fission yeast Schizosaccharomyces pombe is a particularly useful model for studying the function and regulation of genes from higher eukaryotes. The genome of Sc. pombe has been sequenced, and DNA microarray, proteome and transcriptome analyses have been carried out. Among the well-characterized yeast species, Sc. pombe is considered an attractive host for the production of heterologous proteins. Expression vectors for high-level expression in Sc. pombe have been developed and many foreign proteins have been successfully expressed. However, further improvements in the protein-expressing host systems are still required for the production of heterologous proteins involved in post-translationalmodification, metabolism and intracellular trafficking. This minireview focuses on recent advances in heterologous protein production by use of engineered fission-yeast strains.

本文言語英語
ページ(範囲)227-235
ページ数9
ジャーナルBiotechnology and Applied Biochemistry
53
4
DOI
出版ステータス出版済み - 2009

All Science Journal Classification (ASJC) codes

  • バイオテクノロジー
  • バイオエンジニアリング
  • 分子医療
  • 生体医工学
  • 応用微生物学とバイオテクノロジー
  • 創薬
  • プロセス化学およびプロセス工学

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