Production of heterologous proteins using the fission-yeast (Schizosaccharomyces pombe) expression system

Kaoru Takegawa, Hideki Tohda, Mayumi Sasaki, Alimjan Idiris, Takao Ohashi, Hiroyuki Mukaiyama, Yuko Giga-Hama, Hiromichi Kumagai

研究成果: ジャーナルへの寄稿小調査

45 引用 (Scopus)

抄録

The fission yeast Schizosaccharomyces pombe is a particularly useful model for studying the function and regulation of genes from higher eukaryotes. The genome of Sc. pombe has been sequenced, and DNA microarray, proteome and transcriptome analyses have been carried out. Among the well-characterized yeast species, Sc. pombe is considered an attractive host for the production of heterologous proteins. Expression vectors for high-level expression in Sc. pombe have been developed and many foreign proteins have been successfully expressed. However, further improvements in the protein-expressing host systems are still required for the production of heterologous proteins involved in post-translationalmodification, metabolism and intracellular trafficking. This minireview focuses on recent advances in heterologous protein production by use of engineered fission-yeast strains.

元の言語英語
ページ(範囲)227-235
ページ数9
ジャーナルBiotechnology and Applied Biochemistry
53
発行部数4
DOI
出版物ステータス出版済み - 8 3 2009

Fingerprint

Schizosaccharomyces pombe Proteins
Schizosaccharomyces
Yeast
Proteins
Genes
Proteome
Microarrays
Metabolism
Gene Expression Profiling
Microarray Analysis
Oligonucleotide Array Sequence Analysis
Eukaryota
DNA
Yeasts
Genome

All Science Journal Classification (ASJC) codes

  • Biotechnology
  • Bioengineering
  • Molecular Medicine
  • Biomedical Engineering
  • Applied Microbiology and Biotechnology
  • Drug Discovery
  • Process Chemistry and Technology

これを引用

Production of heterologous proteins using the fission-yeast (Schizosaccharomyces pombe) expression system. / Takegawa, Kaoru; Tohda, Hideki; Sasaki, Mayumi; Idiris, Alimjan; Ohashi, Takao; Mukaiyama, Hiroyuki; Giga-Hama, Yuko; Kumagai, Hiromichi.

:: Biotechnology and Applied Biochemistry, 巻 53, 番号 4, 03.08.2009, p. 227-235.

研究成果: ジャーナルへの寄稿小調査

Takegawa, K, Tohda, H, Sasaki, M, Idiris, A, Ohashi, T, Mukaiyama, H, Giga-Hama, Y & Kumagai, H 2009, 'Production of heterologous proteins using the fission-yeast (Schizosaccharomyces pombe) expression system', Biotechnology and Applied Biochemistry, 巻. 53, 番号 4, pp. 227-235. https://doi.org/10.1042/BA20090048
Takegawa, Kaoru ; Tohda, Hideki ; Sasaki, Mayumi ; Idiris, Alimjan ; Ohashi, Takao ; Mukaiyama, Hiroyuki ; Giga-Hama, Yuko ; Kumagai, Hiromichi. / Production of heterologous proteins using the fission-yeast (Schizosaccharomyces pombe) expression system. :: Biotechnology and Applied Biochemistry. 2009 ; 巻 53, 番号 4. pp. 227-235.
@article{1c886740e49d49758d840e5f30debbbe,
title = "Production of heterologous proteins using the fission-yeast (Schizosaccharomyces pombe) expression system",
abstract = "The fission yeast Schizosaccharomyces pombe is a particularly useful model for studying the function and regulation of genes from higher eukaryotes. The genome of Sc. pombe has been sequenced, and DNA microarray, proteome and transcriptome analyses have been carried out. Among the well-characterized yeast species, Sc. pombe is considered an attractive host for the production of heterologous proteins. Expression vectors for high-level expression in Sc. pombe have been developed and many foreign proteins have been successfully expressed. However, further improvements in the protein-expressing host systems are still required for the production of heterologous proteins involved in post-translationalmodification, metabolism and intracellular trafficking. This minireview focuses on recent advances in heterologous protein production by use of engineered fission-yeast strains.",
author = "Kaoru Takegawa and Hideki Tohda and Mayumi Sasaki and Alimjan Idiris and Takao Ohashi and Hiroyuki Mukaiyama and Yuko Giga-Hama and Hiromichi Kumagai",
year = "2009",
month = "8",
day = "3",
doi = "10.1042/BA20090048",
language = "English",
volume = "53",
pages = "227--235",
journal = "Biotechnology and Applied Biochemistry",
issn = "0885-4513",
publisher = "Wiley-Blackwell",
number = "4",

}

TY - JOUR

T1 - Production of heterologous proteins using the fission-yeast (Schizosaccharomyces pombe) expression system

AU - Takegawa, Kaoru

AU - Tohda, Hideki

AU - Sasaki, Mayumi

AU - Idiris, Alimjan

AU - Ohashi, Takao

AU - Mukaiyama, Hiroyuki

AU - Giga-Hama, Yuko

AU - Kumagai, Hiromichi

PY - 2009/8/3

Y1 - 2009/8/3

N2 - The fission yeast Schizosaccharomyces pombe is a particularly useful model for studying the function and regulation of genes from higher eukaryotes. The genome of Sc. pombe has been sequenced, and DNA microarray, proteome and transcriptome analyses have been carried out. Among the well-characterized yeast species, Sc. pombe is considered an attractive host for the production of heterologous proteins. Expression vectors for high-level expression in Sc. pombe have been developed and many foreign proteins have been successfully expressed. However, further improvements in the protein-expressing host systems are still required for the production of heterologous proteins involved in post-translationalmodification, metabolism and intracellular trafficking. This minireview focuses on recent advances in heterologous protein production by use of engineered fission-yeast strains.

AB - The fission yeast Schizosaccharomyces pombe is a particularly useful model for studying the function and regulation of genes from higher eukaryotes. The genome of Sc. pombe has been sequenced, and DNA microarray, proteome and transcriptome analyses have been carried out. Among the well-characterized yeast species, Sc. pombe is considered an attractive host for the production of heterologous proteins. Expression vectors for high-level expression in Sc. pombe have been developed and many foreign proteins have been successfully expressed. However, further improvements in the protein-expressing host systems are still required for the production of heterologous proteins involved in post-translationalmodification, metabolism and intracellular trafficking. This minireview focuses on recent advances in heterologous protein production by use of engineered fission-yeast strains.

UR - http://www.scopus.com/inward/record.url?scp=67749131163&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=67749131163&partnerID=8YFLogxK

U2 - 10.1042/BA20090048

DO - 10.1042/BA20090048

M3 - Short survey

VL - 53

SP - 227

EP - 235

JO - Biotechnology and Applied Biochemistry

JF - Biotechnology and Applied Biochemistry

SN - 0885-4513

IS - 4

ER -