Production of isoflavonoids in callus cultures of pueraria candollei var. mirifica

Latiporn Udomsuk, Kanokwan Jarukamjorn, Hiroyuki Tanaka, Waraporn Putalun

研究成果: ジャーナルへの寄稿記事

7 引用 (Scopus)

抄録

Pueraria candollei Wall, ex Benth. var. mirifica (Airy Shaw & Suvat.) Niyomdham was investigated for callus induction using Murashige and Skoog (MS) medium containing different plant growth regulators. After 8 weeks of culture, 66-100% of leaf or stem expiants formed calli. Calli from stem expiants cultured on MS medium supplemented with 0.5 mg/1 thidiazuron (TDZ) gave the maximum of shoot induction (16%) and the highest level of total isoflavonoids [(50.39 ± 7.06) mg/g dry wt], which was 7-fold higher than that of the native tuber [(7.04 ± 0.29) mg/g dry wt]. These results suggest that addition of TDZ to the culture medium markedly enhances the production of isoflavonoids in calli induced from stem expiants of P. candollei var. mirifica.

元の言語英語
ページ(範囲)239-243
ページ数5
ジャーナルZeitschrift fur Naturforschung - Section C Journal of Biosciences
64
発行部数3-4
DOI
出版物ステータス出版済み - 1 1 2009

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Pueraria
Bony Callus
Plant Growth Regulators
Culture Media
thidiazuron

All Science Journal Classification (ASJC) codes

  • Biochemistry, Genetics and Molecular Biology(all)

これを引用

Production of isoflavonoids in callus cultures of pueraria candollei var. mirifica. / Udomsuk, Latiporn; Jarukamjorn, Kanokwan; Tanaka, Hiroyuki; Putalun, Waraporn.

:: Zeitschrift fur Naturforschung - Section C Journal of Biosciences, 巻 64, 番号 3-4, 01.01.2009, p. 239-243.

研究成果: ジャーナルへの寄稿記事

Udomsuk, Latiporn ; Jarukamjorn, Kanokwan ; Tanaka, Hiroyuki ; Putalun, Waraporn. / Production of isoflavonoids in callus cultures of pueraria candollei var. mirifica. :: Zeitschrift fur Naturforschung - Section C Journal of Biosciences. 2009 ; 巻 64, 番号 3-4. pp. 239-243.
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abstract = "Pueraria candollei Wall, ex Benth. var. mirifica (Airy Shaw & Suvat.) Niyomdham was investigated for callus induction using Murashige and Skoog (MS) medium containing different plant growth regulators. After 8 weeks of culture, 66-100{\%} of leaf or stem expiants formed calli. Calli from stem expiants cultured on MS medium supplemented with 0.5 mg/1 thidiazuron (TDZ) gave the maximum of shoot induction (16{\%}) and the highest level of total isoflavonoids [(50.39 ± 7.06) mg/g dry wt], which was 7-fold higher than that of the native tuber [(7.04 ± 0.29) mg/g dry wt]. These results suggest that addition of TDZ to the culture medium markedly enhances the production of isoflavonoids in calli induced from stem expiants of P. candollei var. mirifica.",
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