Production of l-theanine using glutaminase encapsulated in carbon-coated mesoporous silica with high pH stability

Tetsuji Itoh, Yasuto Hoshikawa, Shun Ichi Matsuura, Junko Mizuguchi, Hiroyuki Arafune, Taka Aki Hanaoka, Fujio Mizukami, Akari Hayashi, Hirotomo Nishihara, Takashi Kyotani

研究成果: Contribution to journalArticle査読

24 被引用数 (Scopus)

抄録

A new enzymatic process for production of l-theanine was presented using glutaminase combined with immobilization technique on carbon-coated mesoporous silica (carbon/SBA-15). For the attempt to enhance the durability against high pH conditions, the pore surface of mesoporous silica SBA-15 was coated with a thin layer of carbon. Carbon coating was done by addition of 2,3-dihydroxynaphthalene (DN) on the pore surface, followed by a dehydration reaction between surface silanol groups in SBA-15 and hydroxyl groups of DN molecules, and further carbonization of DN. A glutaminase was confined in nanospace (about 5. nm) of carbon/SBA-15. The resulting product, glutaminase/carbon/SBA-15, successfully catalyzed the reaction for production of l-theanine under high pH conditions.

本文言語英語
ページ(範囲)207-214
ページ数8
ジャーナルBiochemical Engineering Journal
68
DOI
出版ステータス出版済み - 10 15 2012

All Science Journal Classification (ASJC) codes

  • バイオテクノロジー
  • 環境工学
  • バイオエンジニアリング
  • 生体医工学

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