Production of l-theanine using glutaminase encapsulated in carbon-coated mesoporous silica with high pH stability

Tetsuji Itoh, Yasuto Hoshikawa, Shun Ichi Matsuura, Junko Mizuguchi, Hiroyuki Arafune, Taka Aki Hanaoka, Fujio Mizukami, Akari Hayashi, Hirotomo Nishihara, Takashi Kyotani

研究成果: ジャーナルへの寄稿記事

19 引用 (Scopus)

抜粋

A new enzymatic process for production of l-theanine was presented using glutaminase combined with immobilization technique on carbon-coated mesoporous silica (carbon/SBA-15). For the attempt to enhance the durability against high pH conditions, the pore surface of mesoporous silica SBA-15 was coated with a thin layer of carbon. Carbon coating was done by addition of 2,3-dihydroxynaphthalene (DN) on the pore surface, followed by a dehydration reaction between surface silanol groups in SBA-15 and hydroxyl groups of DN molecules, and further carbonization of DN. A glutaminase was confined in nanospace (about 5. nm) of carbon/SBA-15. The resulting product, glutaminase/carbon/SBA-15, successfully catalyzed the reaction for production of l-theanine under high pH conditions.

元の言語英語
ページ(範囲)207-214
ページ数8
ジャーナルBiochemical Engineering Journal
68
DOI
出版物ステータス出版済み - 10 15 2012

    フィンガープリント

All Science Journal Classification (ASJC) codes

  • Biotechnology
  • Environmental Engineering
  • Bioengineering
  • Biomedical Engineering

これを引用

Itoh, T., Hoshikawa, Y., Matsuura, S. I., Mizuguchi, J., Arafune, H., Hanaoka, T. A., ... Kyotani, T. (2012). Production of l-theanine using glutaminase encapsulated in carbon-coated mesoporous silica with high pH stability. Biochemical Engineering Journal, 68, 207-214. https://doi.org/10.1016/j.bej.2012.07.012