Callus tissue from petioles of Salvia miltiorrhiza was obtained on Murashige and Skoog medium supplemented with indole-3-butyric acid and 6-benzylaminopurine. When the calli were subcultured, adventitious shoots formed. These shoots developed into normal plantlets with roots when transferred to hormone-free Murashige and Skoog medium. Clonal micropropagation was established by shoot tip culture and was maintained on Murashige and Skoog medium supplemented with kinetin and gibberellic acid A3. Callus that was induced and maintained on supplements of 2,4-dichlorophenoxyacetic acid and 6-benzylaminopurine in the absence of light produced rosmarinic acid (1.24% dry wt) and lithospermic acid B (0.10% dry wt). Higher concentration levels of rosmarinic acid (6.96% dry wt) and lithospermic acid B (6.05% dry wt) were formed in the leaves of 15-week-old plants that were generated by micropropagation.
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