Production of lithospermic acid B and rosmarinic acid in callus tissue and regenerated plantlets of salvia miltiorrhiza

Satoshi Morimoto, Yuri Goto, Yukihiro Shoyama

研究成果: Contribution to journalArticle査読

50 被引用数 (Scopus)

抄録

Callus tissue from petioles of Salvia miltiorrhiza was obtained on Murashige and Skoog medium supplemented with indole-3-butyric acid and 6-benzylaminopurine. When the calli were subcultured, adventitious shoots formed. These shoots developed into normal plantlets with roots when transferred to hormone-free Murashige and Skoog medium. Clonal micropropagation was established by shoot tip culture and was maintained on Murashige and Skoog medium supplemented with kinetin and gibberellic acid A3. Callus that was induced and maintained on supplements of 2,4-dichlorophenoxyacetic acid and 6-benzylaminopurine in the absence of light produced rosmarinic acid (1.24% dry wt) and lithospermic acid B (0.10% dry wt). Higher concentration levels of rosmarinic acid (6.96% dry wt) and lithospermic acid B (6.05% dry wt) were formed in the leaves of 15-week-old plants that were generated by micropropagation.

本文言語英語
ページ(範囲)817-823
ページ数7
ジャーナルJournal of Natural Products
57
6
DOI
出版ステータス出版済み - 6 1 1994

All Science Journal Classification (ASJC) codes

  • Analytical Chemistry
  • Molecular Medicine
  • Pharmacology
  • Pharmaceutical Science
  • Drug Discovery
  • Complementary and alternative medicine
  • Organic Chemistry

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