Production of Monoclonal Antibodies Against Leptospiral Genus-Specific Protein Antigen and Localization of the Antigen by Immunoelectron Microscopy

Noritsugu Sakamoto, Etsuro Ono, Hiroshi Kida, Ryo Yanagawa

研究成果: ジャーナルへの寄稿記事

10 引用 (Scopus)

抄録

Monoclonal antibodies were first produced against a leptospiral genus-specific protein antigen (GP-Ag) isolated from Leptospira interrogans serovar kremastos strain Kyoto. The representative monoclonal antibody (GP-7) did not agglutinate kremastos Kyoto but reacted with Triton X-100 treated kreamastos Kyoto, andamana, patoc and Urawa in enzyme-linked immunosorbent assay (ELISA), indicating that the monoclonal antibody recognized the genus-specific determinant of GP-Ag. The localization of GP-Ag was investigated by immunoelectron microscopy with the iodinated antibody and peroxidase-labeled antibody. The genus-specific determinant of GP-Ag recognized by GP-7 was located on the subsurface of the leptospiral envelope.

元の言語英語
ページ(範囲)557-563
ページ数7
ジャーナルZentralblatt fur Bakteriologie Mikrobiologie und Hygiene - Abt. 1 Orig. A
259
発行部数4
DOI
出版物ステータス出版済み - 1 1 1985
外部発表Yes

Fingerprint

Immunoelectron Microscopy
Monoclonal Antibodies
Antigens
Leptospira interrogans
Proteins
Antibodies
Octoxynol
Peroxidase
Enzyme-Linked Immunosorbent Assay
GP 7

All Science Journal Classification (ASJC) codes

  • Immunology

これを引用

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abstract = "Monoclonal antibodies were first produced against a leptospiral genus-specific protein antigen (GP-Ag) isolated from Leptospira interrogans serovar kremastos strain Kyoto. The representative monoclonal antibody (GP-7) did not agglutinate kremastos Kyoto but reacted with Triton X-100 treated kreamastos Kyoto, andamana, patoc and Urawa in enzyme-linked immunosorbent assay (ELISA), indicating that the monoclonal antibody recognized the genus-specific determinant of GP-Ag. The localization of GP-Ag was investigated by immunoelectron microscopy with the iodinated antibody and peroxidase-labeled antibody. The genus-specific determinant of GP-Ag recognized by GP-7 was located on the subsurface of the leptospiral envelope.",
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AU - Ono, Etsuro

AU - Kida, Hiroshi

AU - Yanagawa, Ryo

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AB - Monoclonal antibodies were first produced against a leptospiral genus-specific protein antigen (GP-Ag) isolated from Leptospira interrogans serovar kremastos strain Kyoto. The representative monoclonal antibody (GP-7) did not agglutinate kremastos Kyoto but reacted with Triton X-100 treated kreamastos Kyoto, andamana, patoc and Urawa in enzyme-linked immunosorbent assay (ELISA), indicating that the monoclonal antibody recognized the genus-specific determinant of GP-Ag. The localization of GP-Ag was investigated by immunoelectron microscopy with the iodinated antibody and peroxidase-labeled antibody. The genus-specific determinant of GP-Ag recognized by GP-7 was located on the subsurface of the leptospiral envelope.

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