Production of monoclonal antibodies specific to major allergens of Cryptomeria japonica pollen

K. Honda, H. Shirae, K. Miwa, S. Matsushita, K. Makishima, Y. Nishimura, T. Sasazuki

研究成果: Contribution to journalArticle査読

3 被引用数 (Scopus)

抄録

Monoclonal antibodies (MAbs) directed against Cryptomeria japonica pollen antigen (CPAg) were prepared. CPAg was precipitated with these MAbs and both MAbs CPA7 (IgG(2a)) and CPA9 (IgG1) recognized two major glycoproteins (m=41kDa and 46 kDa) of CPAg. MAb CPA7 or CPA9 was coupled to CNBr activated sepharose and affinity purification of the major allergen of CPAg from the crude extract was performed. The affinity purified CPAg bound IgE antibody present in patients with Cedar pollinosis.

本文言語英語
ページ(範囲)361-366
ページ数6
ジャーナルHybridoma
11
3
DOI
出版ステータス出版済み - 1992

All Science Journal Classification (ASJC) codes

  • 免疫学
  • 遺伝学

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