Production of monoclonal antibody against a major purgative component, sennoside A, its characterization and ELISA

Osamu Morinaga, Hiroyuki Tanaka, Yukihiro Shoyama

研究成果: ジャーナルへの寄稿記事

32 引用 (Scopus)

抄録

For immunization, sennoside A was conjugated with bovine serum albumin. The hapten number in an antigen conjugate was determined to be five by matrix-assisted laser desorption/ionization TOF mass spectrometry. A hybridoma secreting monoclonal antibody against sennoside A was produced by fusing splenocytes immunized with sennoside A-bovine serum albumin and a hypoxantine-aminopterin-thymidine (HAT)-sensitive mouse myeloma cell line, P3-X63-Ag8-653. Weak cross-reactivities occurred with sennoside B which is a stereochemical isomer, and a monomer of sennoside A, rhein, but no cross-reactivities were observed with other related anthraquinones and phenolics. The full range of the assay extends from 20 to 200 ng ml-1 of sennoside A. Good correlation of sennoside A concentrations in crude extract of rhubarb between ELISA and HPLC methods was obtained. The contents of sennoside A in various rhubarb roots were assayed by the newly established ELISA indicating a good agreement with the previous reports.

元の言語英語
ページ(範囲)1109-1113
ページ数5
ジャーナルAnalyst
125
発行部数6
DOI
出版物ステータス出版済み - 7 1 2000

Fingerprint

Cathartics
Immunization
Monoclonal antibodies
Antigens
Isomers
antibody
Ionization
Mass spectrometry
serum
Assays
Desorption
Monomers
Enzyme-Linked Immunosorbent Assay
Monoclonal Antibodies
Cells
immunization
Lasers
antigen
desorption
ionization

All Science Journal Classification (ASJC) codes

  • Analytical Chemistry
  • Biochemistry
  • Environmental Chemistry
  • Spectroscopy
  • Electrochemistry

これを引用

Production of monoclonal antibody against a major purgative component, sennoside A, its characterization and ELISA. / Morinaga, Osamu; Tanaka, Hiroyuki; Shoyama, Yukihiro.

:: Analyst, 巻 125, 番号 6, 01.07.2000, p. 1109-1113.

研究成果: ジャーナルへの寄稿記事

Morinaga, Osamu ; Tanaka, Hiroyuki ; Shoyama, Yukihiro. / Production of monoclonal antibody against a major purgative component, sennoside A, its characterization and ELISA. :: Analyst. 2000 ; 巻 125, 番号 6. pp. 1109-1113.
@article{2fcd8e13b9c1445ab3d6798febe5a1db,
title = "Production of monoclonal antibody against a major purgative component, sennoside A, its characterization and ELISA",
abstract = "For immunization, sennoside A was conjugated with bovine serum albumin. The hapten number in an antigen conjugate was determined to be five by matrix-assisted laser desorption/ionization TOF mass spectrometry. A hybridoma secreting monoclonal antibody against sennoside A was produced by fusing splenocytes immunized with sennoside A-bovine serum albumin and a hypoxantine-aminopterin-thymidine (HAT)-sensitive mouse myeloma cell line, P3-X63-Ag8-653. Weak cross-reactivities occurred with sennoside B which is a stereochemical isomer, and a monomer of sennoside A, rhein, but no cross-reactivities were observed with other related anthraquinones and phenolics. The full range of the assay extends from 20 to 200 ng ml-1 of sennoside A. Good correlation of sennoside A concentrations in crude extract of rhubarb between ELISA and HPLC methods was obtained. The contents of sennoside A in various rhubarb roots were assayed by the newly established ELISA indicating a good agreement with the previous reports.",
author = "Osamu Morinaga and Hiroyuki Tanaka and Yukihiro Shoyama",
year = "2000",
month = "7",
day = "1",
doi = "10.1039/b000988l",
language = "English",
volume = "125",
pages = "1109--1113",
journal = "The Analyst",
issn = "0003-2654",
publisher = "Royal Society of Chemistry",
number = "6",

}

TY - JOUR

T1 - Production of monoclonal antibody against a major purgative component, sennoside A, its characterization and ELISA

AU - Morinaga, Osamu

AU - Tanaka, Hiroyuki

AU - Shoyama, Yukihiro

PY - 2000/7/1

Y1 - 2000/7/1

N2 - For immunization, sennoside A was conjugated with bovine serum albumin. The hapten number in an antigen conjugate was determined to be five by matrix-assisted laser desorption/ionization TOF mass spectrometry. A hybridoma secreting monoclonal antibody against sennoside A was produced by fusing splenocytes immunized with sennoside A-bovine serum albumin and a hypoxantine-aminopterin-thymidine (HAT)-sensitive mouse myeloma cell line, P3-X63-Ag8-653. Weak cross-reactivities occurred with sennoside B which is a stereochemical isomer, and a monomer of sennoside A, rhein, but no cross-reactivities were observed with other related anthraquinones and phenolics. The full range of the assay extends from 20 to 200 ng ml-1 of sennoside A. Good correlation of sennoside A concentrations in crude extract of rhubarb between ELISA and HPLC methods was obtained. The contents of sennoside A in various rhubarb roots were assayed by the newly established ELISA indicating a good agreement with the previous reports.

AB - For immunization, sennoside A was conjugated with bovine serum albumin. The hapten number in an antigen conjugate was determined to be five by matrix-assisted laser desorption/ionization TOF mass spectrometry. A hybridoma secreting monoclonal antibody against sennoside A was produced by fusing splenocytes immunized with sennoside A-bovine serum albumin and a hypoxantine-aminopterin-thymidine (HAT)-sensitive mouse myeloma cell line, P3-X63-Ag8-653. Weak cross-reactivities occurred with sennoside B which is a stereochemical isomer, and a monomer of sennoside A, rhein, but no cross-reactivities were observed with other related anthraquinones and phenolics. The full range of the assay extends from 20 to 200 ng ml-1 of sennoside A. Good correlation of sennoside A concentrations in crude extract of rhubarb between ELISA and HPLC methods was obtained. The contents of sennoside A in various rhubarb roots were assayed by the newly established ELISA indicating a good agreement with the previous reports.

UR - http://www.scopus.com/inward/record.url?scp=0034041938&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0034041938&partnerID=8YFLogxK

U2 - 10.1039/b000988l

DO - 10.1039/b000988l

M3 - Article

AN - SCOPUS:0034041938

VL - 125

SP - 1109

EP - 1113

JO - The Analyst

JF - The Analyst

SN - 0003-2654

IS - 6

ER -