Production of polyclonal antibody against madecassoside and development of immunoassay methods for analysis of triterpene glycosides in Centella asiatica

Patcharin Tassanawat, Waraporn Putalun, Gorawit Yusakul, Boonchoo Sritularak, Thaweesak Juengwatanatrakul, Hiroyuki Tanaka

研究成果: ジャーナルへの寄稿記事

3 引用 (Scopus)

抄録

Introduction Centella asiatica (L.) Urban consists of two major triterpene glycosides, asiaticoside (AS) and madecassoside (MA), as active components used for wound healing and enhancing memory. Objective To produce a polyclonal antibody against madecassoside (MA-PAb) and develop enzyme-linked immunosorbent assay (ELISA) and Eastern blotting methods for quantitative analysis of triterpene glycosides in Centella asiatica. Methods An ELISA method was developed using polyclonal antibody against MA. An Eastern blotting method on the PES membrane was established for determination of MA and AS. The immunoassays were validated for sensitivity, precision, specificity and accuracy. Results The prepared MA-PAb shows specificity to MA and AS. The measuring range of triterpene glycosides was 0.39-50 μg/mL using the ELISA method. An Eastern blotting method was developed for determining individual MA and AS, which could be detected in the range of 62.5-500 ng. The limit of detection for MA and AS was 31.25 ng. The two methods developed showed good specificity, precision, and accuracy, and also correlated with high-performance liquid chromatography. Conclusion These immunoassays have several advantages that include high sensitivity as well as being rapid and facile for determination of the triterpene glycosides in C. asiatica.

元の言語英語
ページ(範囲)256-262
ページ数7
ジャーナルPhytochemical Analysis
24
発行部数3
DOI
出版物ステータス出版済み - 5 1 2013

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Centella
Centella asiatica
Triterpenes
Glycosides
immunoassays
polyclonal antibodies
Immunoassay
triterpenoids
Antibody Formation
glycosides
Antibodies
Immunosorbents
enzyme-linked immunosorbent assay
Assays
Enzyme-Linked Immunosorbent Assay
methodology
Enzymes
madecassoside
tissue repair
quantitative analysis

All Science Journal Classification (ASJC) codes

  • Analytical Chemistry
  • Food Science
  • Biochemistry
  • Molecular Medicine
  • Plant Science
  • Drug Discovery
  • Complementary and alternative medicine

これを引用

Production of polyclonal antibody against madecassoside and development of immunoassay methods for analysis of triterpene glycosides in Centella asiatica. / Tassanawat, Patcharin; Putalun, Waraporn; Yusakul, Gorawit; Sritularak, Boonchoo; Juengwatanatrakul, Thaweesak; Tanaka, Hiroyuki.

:: Phytochemical Analysis, 巻 24, 番号 3, 01.05.2013, p. 256-262.

研究成果: ジャーナルへの寄稿記事

Tassanawat, Patcharin ; Putalun, Waraporn ; Yusakul, Gorawit ; Sritularak, Boonchoo ; Juengwatanatrakul, Thaweesak ; Tanaka, Hiroyuki. / Production of polyclonal antibody against madecassoside and development of immunoassay methods for analysis of triterpene glycosides in Centella asiatica. :: Phytochemical Analysis. 2013 ; 巻 24, 番号 3. pp. 256-262.
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AU - Yusakul, Gorawit

AU - Sritularak, Boonchoo

AU - Juengwatanatrakul, Thaweesak

AU - Tanaka, Hiroyuki

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