Production of scFv-Fc fusion protein using genetically manipulated quails

Yoshinori Kawabe, Masamichi Kamihira, Ken ichiro Ono, Kenji Kyogoku, Ken ichi Nishijima, Shinji Iijima

研究成果: Contribution to journalArticle査読

16 被引用数 (Scopus)

抄録

The use of transgenic avian species as a transgenic bioreactor for the production of recombinant proteins has been proposed. In recent years, although various procedures for generating transgenic chickens have been reported, the expression of a useful protein at a commercially feasible level has rarely been attained. In this study, we injected a concentrated retroviral vector into quail embryos to generate genetically manipulated quails that produce recombinant proteins. We found that transgene expression in the whole body at a high level was observed for viral injection into the heart of the developing embryos after a 48-h incubation. For the practical production of a useful protein, a retroviral vector encoding an anti-prion scFv-Fc gene under the control of the β-actin promoter was injected into quail embryos. The quails that hatched stably produced scFv-Fc at a high level in their serum and egg white. The production of scFv-Fc was maintained throughout the breeding period. scFv-Fc purified from the egg white retained the antigen-binding activity. This system exhibited the potential of transgenic quails for the commercial production of recombinant proteins.

本文言語英語
ページ(範囲)297-303
ページ数7
ジャーナルJournal of Bioscience and Bioengineering
102
4
DOI
出版ステータス出版済み - 10 2006
外部発表はい

All Science Journal Classification (ASJC) codes

  • Biotechnology
  • Bioengineering
  • Applied Microbiology and Biotechnology

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