Production, Purification, and Characterization of D-Aminoacylase from Alcaligenes xylosoxydans subsp. Xylosoxydans A-6

Mitsuaki Moriguchi, Kenji Sakai, Yoshiro Miyamoto, Mamoru Wakayama

研究成果: ジャーナルへの寄稿記事

33 引用 (Scopus)

抄録

The best inducers for D-aminoacylase from Alcaligenes xylosoxydans subsp. xylosoxydans A-6(Alcaligenes A-6) were a poor substrate, N-acetyl-γ-methyl-D-leucine, and an inhibitor, N-acetyl-D-alloisoleucine. The enzyme has been homogeneously purified. The molecular weight of the native enzyme was estimated to be 58, 000 by gel filtration. A subunit molecular weight of 52, 000 was measured by SDS-PAGE, indicating that the native protein is a monomer. The isoelectric point was 5.2. The enzyme was specific to the D-isomer and hydrolyzed N-acetyl derivatives of D-leucine, D-phenylalanine, D-norleucine, D-methionine, and D-valine, and also N-formyl, N-butyryl, and N-propionyl derivatives of D-leucine. The Km for N-acetyl-D-leucine was 9.8 mM. The optimum pH and temperature were 7.0 and 50°C, respectively. The stabilities of pH and temperature were 8.1 and 40°C. D-Aminoacylases from three species of the genus Alcaligenes differ in inducer and substrate specificities, but are similar with respect to molecular weight and N-terminal amino acid sequence.

元の言語英語
ページ(範囲)1149-1152
ページ数4
ジャーナルBioscience, biotechnology, and biochemistry
57
発行部数7
DOI
出版物ステータス出版済み - 1 1 1993

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Alcaligenes
Leucine
Purification
Molecular Weight
Molecular weight
Enzymes
Norleucine
Temperature
Isoleucine
Isoelectric Point
Valine
Substrate Specificity
Derivatives
Phenylalanine
Methionine
Gel Chromatography
Polyacrylamide Gel Electrophoresis
Amino Acid Sequence
Substrates
Isomers

All Science Journal Classification (ASJC) codes

  • Biotechnology
  • Analytical Chemistry
  • Biochemistry
  • Applied Microbiology and Biotechnology
  • Molecular Biology
  • Organic Chemistry

これを引用

Production, Purification, and Characterization of D-Aminoacylase from Alcaligenes xylosoxydans subsp. Xylosoxydans A-6. / Moriguchi, Mitsuaki; Sakai, Kenji; Miyamoto, Yoshiro; Wakayama, Mamoru.

:: Bioscience, biotechnology, and biochemistry, 巻 57, 番号 7, 01.01.1993, p. 1149-1152.

研究成果: ジャーナルへの寄稿記事

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