Production, Purification, and Characterization of D-Aminoacylase from Alcaligenes xylosoxydans subsp. Xylosoxydans A-6

Mitsuaki Moriguchi, Kenji Sakai, Yoshiro Miyamoto, Mamoru Wakayama

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The best inducers for D-aminoacylase from Alcaligenes xylosoxydans subsp. xylosoxydans A-6(Alcaligenes A-6) were a poor substrate, N-acetyl-γ-methyl-D-leucine, and an inhibitor, N-acetyl-D-alloisoleucine. The enzyme has been homogeneously purified. The molecular weight of the native enzyme was estimated to be 58, 000 by gel filtration. A subunit molecular weight of 52, 000 was measured by SDS-PAGE, indicating that the native protein is a monomer. The isoelectric point was 5.2. The enzyme was specific to the D-isomer and hydrolyzed N-acetyl derivatives of D-leucine, D-phenylalanine, D-norleucine, D-methionine, and D-valine, and also N-formyl, N-butyryl, and N-propionyl derivatives of D-leucine. The Km for N-acetyl-D-leucine was 9.8 mM. The optimum pH and temperature were 7.0 and 50°C, respectively. The stabilities of pH and temperature were 8.1 and 40°C. D-Aminoacylases from three species of the genus Alcaligenes differ in inducer and substrate specificities, but are similar with respect to molecular weight and N-terminal amino acid sequence.

元の言語英語
ページ(範囲)1149-1152
ページ数4
ジャーナルBioscience, biotechnology, and biochemistry
57
発行部数7
DOI
出版物ステータス出版済み - 1 1 1993
外部発表Yes

All Science Journal Classification (ASJC) codes

  • Biotechnology
  • Analytical Chemistry
  • Biochemistry
  • Applied Microbiology and Biotechnology
  • Molecular Biology
  • Organic Chemistry

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