Profiling of autoantibodies in sera of pancreatic cancer patients

Yosuke Nagayoshi, Masafumi Nakamura, Kazuhiro Matsuoka, Ohtsuka Takao, Yasuhisa Mori, Hiroshi Kono, Teppei Aso, Noboru Ideno, Shunichi Takahata, Akihide Ryo, Hiroyuki Takeda, Tetsuhide Ito, Yoshinao Oda, Yaeta Endo, Tatsuya Sawasaki, Masao Tanaka

研究成果: ジャーナルへの寄稿記事

14 引用 (Scopus)

抄録

Background. Although autoantibodies to cancer antigens are candidates for biomarkers, no comprehensive studies to detect cancer-specific antibodies have been performed. This study identified autoantibodies in the sera of pancreatic cancer (PC) patients using proteomics based on a wheat germ cell-free protein production system. Methods. We constructed a biotinylated protein library of 2,183 genes. Interactions between biotinylated proteins and serum antibodies were detected by AlphaScreen® assay. Relative luminescence signals of each protein in 37 PC patients and 20 healthy controls were measured, and their sensitivity and specificity for PC were calculated. Results. Luminescence signals of nine proteins were significantly higher than those of healthy controls, with calcium and integrin binding 1 (CIB1) protein showing the greatest significance (p = 0.002). Sensitivity, specificity, positive predictive value and negative predictive value of CIB1 autoantibody alone for PC were 76, 70, 82, and 61 %, respectively, and 97, 35, 74, and 88 %, respectively, when the four most significant proteins were combined. Presence of these autoantibodies did not vary significantly with other clinicopathological characteristics. Conclusion. Several autoantibodies, including CIB1, are potential biomarkers for PC.

元の言語英語
ジャーナルAnnals of Surgical Oncology
21
発行部数SUPPL. 3
DOI
出版物ステータス出版済み - 1 1 2014

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Pancreatic Neoplasms
Autoantibodies
Integrins
Serum
Luminescence
Proteins
Calcium
Biomarkers
Sensitivity and Specificity
Antibodies
Germ Cells
Proteomics
Triticum
Blood Proteins
Neoplasms
Carrier Proteins
Antigens
Genes

All Science Journal Classification (ASJC) codes

  • Surgery
  • Oncology

これを引用

Profiling of autoantibodies in sera of pancreatic cancer patients. / Nagayoshi, Yosuke; Nakamura, Masafumi; Matsuoka, Kazuhiro; Takao, Ohtsuka; Mori, Yasuhisa; Kono, Hiroshi; Aso, Teppei; Ideno, Noboru; Takahata, Shunichi; Ryo, Akihide; Takeda, Hiroyuki; Ito, Tetsuhide; Oda, Yoshinao; Endo, Yaeta; Sawasaki, Tatsuya; Tanaka, Masao.

:: Annals of Surgical Oncology, 巻 21, 番号 SUPPL. 3, 01.01.2014.

研究成果: ジャーナルへの寄稿記事

Nagayoshi, Y, Nakamura, M, Matsuoka, K, Takao, O, Mori, Y, Kono, H, Aso, T, Ideno, N, Takahata, S, Ryo, A, Takeda, H, Ito, T, Oda, Y, Endo, Y, Sawasaki, T & Tanaka, M 2014, 'Profiling of autoantibodies in sera of pancreatic cancer patients', Annals of Surgical Oncology, 巻. 21, 番号 SUPPL. 3. https://doi.org/10.1245/s10434-014-3574-0
Nagayoshi, Yosuke ; Nakamura, Masafumi ; Matsuoka, Kazuhiro ; Takao, Ohtsuka ; Mori, Yasuhisa ; Kono, Hiroshi ; Aso, Teppei ; Ideno, Noboru ; Takahata, Shunichi ; Ryo, Akihide ; Takeda, Hiroyuki ; Ito, Tetsuhide ; Oda, Yoshinao ; Endo, Yaeta ; Sawasaki, Tatsuya ; Tanaka, Masao. / Profiling of autoantibodies in sera of pancreatic cancer patients. :: Annals of Surgical Oncology. 2014 ; 巻 21, 番号 SUPPL. 3.
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abstract = "Background. Although autoantibodies to cancer antigens are candidates for biomarkers, no comprehensive studies to detect cancer-specific antibodies have been performed. This study identified autoantibodies in the sera of pancreatic cancer (PC) patients using proteomics based on a wheat germ cell-free protein production system. Methods. We constructed a biotinylated protein library of 2,183 genes. Interactions between biotinylated proteins and serum antibodies were detected by AlphaScreen{\circledR} assay. Relative luminescence signals of each protein in 37 PC patients and 20 healthy controls were measured, and their sensitivity and specificity for PC were calculated. Results. Luminescence signals of nine proteins were significantly higher than those of healthy controls, with calcium and integrin binding 1 (CIB1) protein showing the greatest significance (p = 0.002). Sensitivity, specificity, positive predictive value and negative predictive value of CIB1 autoantibody alone for PC were 76, 70, 82, and 61 {\%}, respectively, and 97, 35, 74, and 88 {\%}, respectively, when the four most significant proteins were combined. Presence of these autoantibodies did not vary significantly with other clinicopathological characteristics. Conclusion. Several autoantibodies, including CIB1, are potential biomarkers for PC.",
author = "Yosuke Nagayoshi and Masafumi Nakamura and Kazuhiro Matsuoka and Ohtsuka Takao and Yasuhisa Mori and Hiroshi Kono and Teppei Aso and Noboru Ideno and Shunichi Takahata and Akihide Ryo and Hiroyuki Takeda and Tetsuhide Ito and Yoshinao Oda and Yaeta Endo and Tatsuya Sawasaki and Masao Tanaka",
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T1 - Profiling of autoantibodies in sera of pancreatic cancer patients

AU - Nagayoshi, Yosuke

AU - Nakamura, Masafumi

AU - Matsuoka, Kazuhiro

AU - Takao, Ohtsuka

AU - Mori, Yasuhisa

AU - Kono, Hiroshi

AU - Aso, Teppei

AU - Ideno, Noboru

AU - Takahata, Shunichi

AU - Ryo, Akihide

AU - Takeda, Hiroyuki

AU - Ito, Tetsuhide

AU - Oda, Yoshinao

AU - Endo, Yaeta

AU - Sawasaki, Tatsuya

AU - Tanaka, Masao

PY - 2014/1/1

Y1 - 2014/1/1

N2 - Background. Although autoantibodies to cancer antigens are candidates for biomarkers, no comprehensive studies to detect cancer-specific antibodies have been performed. This study identified autoantibodies in the sera of pancreatic cancer (PC) patients using proteomics based on a wheat germ cell-free protein production system. Methods. We constructed a biotinylated protein library of 2,183 genes. Interactions between biotinylated proteins and serum antibodies were detected by AlphaScreen® assay. Relative luminescence signals of each protein in 37 PC patients and 20 healthy controls were measured, and their sensitivity and specificity for PC were calculated. Results. Luminescence signals of nine proteins were significantly higher than those of healthy controls, with calcium and integrin binding 1 (CIB1) protein showing the greatest significance (p = 0.002). Sensitivity, specificity, positive predictive value and negative predictive value of CIB1 autoantibody alone for PC were 76, 70, 82, and 61 %, respectively, and 97, 35, 74, and 88 %, respectively, when the four most significant proteins were combined. Presence of these autoantibodies did not vary significantly with other clinicopathological characteristics. Conclusion. Several autoantibodies, including CIB1, are potential biomarkers for PC.

AB - Background. Although autoantibodies to cancer antigens are candidates for biomarkers, no comprehensive studies to detect cancer-specific antibodies have been performed. This study identified autoantibodies in the sera of pancreatic cancer (PC) patients using proteomics based on a wheat germ cell-free protein production system. Methods. We constructed a biotinylated protein library of 2,183 genes. Interactions between biotinylated proteins and serum antibodies were detected by AlphaScreen® assay. Relative luminescence signals of each protein in 37 PC patients and 20 healthy controls were measured, and their sensitivity and specificity for PC were calculated. Results. Luminescence signals of nine proteins were significantly higher than those of healthy controls, with calcium and integrin binding 1 (CIB1) protein showing the greatest significance (p = 0.002). Sensitivity, specificity, positive predictive value and negative predictive value of CIB1 autoantibody alone for PC were 76, 70, 82, and 61 %, respectively, and 97, 35, 74, and 88 %, respectively, when the four most significant proteins were combined. Presence of these autoantibodies did not vary significantly with other clinicopathological characteristics. Conclusion. Several autoantibodies, including CIB1, are potential biomarkers for PC.

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