抄録
Background. Although autoantibodies to cancer antigens are candidates for biomarkers, no comprehensive studies to detect cancer-specific antibodies have been performed. This study identified autoantibodies in the sera of pancreatic cancer (PC) patients using proteomics based on a wheat germ cell-free protein production system. Methods. We constructed a biotinylated protein library of 2,183 genes. Interactions between biotinylated proteins and serum antibodies were detected by AlphaScreen® assay. Relative luminescence signals of each protein in 37 PC patients and 20 healthy controls were measured, and their sensitivity and specificity for PC were calculated. Results. Luminescence signals of nine proteins were significantly higher than those of healthy controls, with calcium and integrin binding 1 (CIB1) protein showing the greatest significance (p = 0.002). Sensitivity, specificity, positive predictive value and negative predictive value of CIB1 autoantibody alone for PC were 76, 70, 82, and 61 %, respectively, and 97, 35, 74, and 88 %, respectively, when the four most significant proteins were combined. Presence of these autoantibodies did not vary significantly with other clinicopathological characteristics. Conclusion. Several autoantibodies, including CIB1, are potential biomarkers for PC.
| 元の言語 | 英語 |
|---|---|
| ジャーナル | Annals of Surgical Oncology |
| 巻 | 21 |
| 発行部数 | SUPPL. 3 |
| DOI | |
| 出版物ステータス | 出版済み - 1 1 2014 |
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All Science Journal Classification (ASJC) codes
- Surgery
- Oncology
これを引用
Profiling of autoantibodies in sera of pancreatic cancer patients. / Nagayoshi, Yosuke; Nakamura, Masafumi; Matsuoka, Kazuhiro; Takao, Ohtsuka; Mori, Yasuhisa; Kono, Hiroshi; Aso, Teppei; Ideno, Noboru; Takahata, Shunichi; Ryo, Akihide; Takeda, Hiroyuki; Ito, Tetsuhide; Oda, Yoshinao; Endo, Yaeta; Sawasaki, Tatsuya; Tanaka, Masao.
：: Annals of Surgical Oncology, 巻 21, 番号 SUPPL. 3, 01.01.2014.研究成果: ジャーナルへの寄稿 › 記事
}
TY - JOUR
T1 - Profiling of autoantibodies in sera of pancreatic cancer patients
AU - Nagayoshi, Yosuke
AU - Nakamura, Masafumi
AU - Matsuoka, Kazuhiro
AU - Takao, Ohtsuka
AU - Mori, Yasuhisa
AU - Kono, Hiroshi
AU - Aso, Teppei
AU - Ideno, Noboru
AU - Takahata, Shunichi
AU - Ryo, Akihide
AU - Takeda, Hiroyuki
AU - Ito, Tetsuhide
AU - Oda, Yoshinao
AU - Endo, Yaeta
AU - Sawasaki, Tatsuya
AU - Tanaka, Masao
PY - 2014/1/1
Y1 - 2014/1/1
N2 - Background. Although autoantibodies to cancer antigens are candidates for biomarkers, no comprehensive studies to detect cancer-specific antibodies have been performed. This study identified autoantibodies in the sera of pancreatic cancer (PC) patients using proteomics based on a wheat germ cell-free protein production system. Methods. We constructed a biotinylated protein library of 2,183 genes. Interactions between biotinylated proteins and serum antibodies were detected by AlphaScreen® assay. Relative luminescence signals of each protein in 37 PC patients and 20 healthy controls were measured, and their sensitivity and specificity for PC were calculated. Results. Luminescence signals of nine proteins were significantly higher than those of healthy controls, with calcium and integrin binding 1 (CIB1) protein showing the greatest significance (p = 0.002). Sensitivity, specificity, positive predictive value and negative predictive value of CIB1 autoantibody alone for PC were 76, 70, 82, and 61 %, respectively, and 97, 35, 74, and 88 %, respectively, when the four most significant proteins were combined. Presence of these autoantibodies did not vary significantly with other clinicopathological characteristics. Conclusion. Several autoantibodies, including CIB1, are potential biomarkers for PC.
AB - Background. Although autoantibodies to cancer antigens are candidates for biomarkers, no comprehensive studies to detect cancer-specific antibodies have been performed. This study identified autoantibodies in the sera of pancreatic cancer (PC) patients using proteomics based on a wheat germ cell-free protein production system. Methods. We constructed a biotinylated protein library of 2,183 genes. Interactions between biotinylated proteins and serum antibodies were detected by AlphaScreen® assay. Relative luminescence signals of each protein in 37 PC patients and 20 healthy controls were measured, and their sensitivity and specificity for PC were calculated. Results. Luminescence signals of nine proteins were significantly higher than those of healthy controls, with calcium and integrin binding 1 (CIB1) protein showing the greatest significance (p = 0.002). Sensitivity, specificity, positive predictive value and negative predictive value of CIB1 autoantibody alone for PC were 76, 70, 82, and 61 %, respectively, and 97, 35, 74, and 88 %, respectively, when the four most significant proteins were combined. Presence of these autoantibodies did not vary significantly with other clinicopathological characteristics. Conclusion. Several autoantibodies, including CIB1, are potential biomarkers for PC.
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U2 - 10.1245/s10434-014-3574-0
DO - 10.1245/s10434-014-3574-0
M3 - Article
VL - 21
JO - Annals of Surgical Oncology
JF - Annals of Surgical Oncology
SN - 1068-9265
IS - SUPPL. 3
ER -