Proliferative regulation in the cornea and lens

Kazuhiko Yoshida, Takayuki Harada, Chikako Harada, Satoru Kase, Hiromi Ikeda, Masaharu Sakai, Shinzo Nishi, Junko Imaki, Keiko Nakayama, Hiroyasu Nagahama, Keiichi Nakayama, Shigeaki Ohno

研究成果: Contribution to journalReview article

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PURPOSE: To examine the mechanism of regulation of proliferation in epithelial scraped cornea and the developing lens. METHOD AND RESULTS: C57B16 mouse, p27 (KIP 1)-/- mice, Skp2-/- mice and Skp2-/-/p27 (KIP 1)-/- double knockout mice were examined by immunocytochemistry using anti-p27 (KIP1) antibody, and cells in the "S" phase of DNA synthesis were analyzed by immunocytochemistry using anti-BrdU antibody. The p 27 (KIP 1) was expressed in basal cells of the central and peripheral region of the cornea and limbus. This expression was not detected 24 hr after epithelial scraping, when there were many cells in the "S" phase of DNA synthesis in the corneal epithelium. There was no obvious difference in the thickness and anti-BrdU staining in the corneal epithelium of p 27(KIP 1)-/- mice from that of controls. 24 hr after the epithelial scraping in the Skp 2-/- mice, the corneal epithelium was thinner than in wild-type mice and had many p 27(KIP 1) positive cells and few BrdU positive cells. In contrast, 24 hr after the epithelial scraping in the Skp 2-/-/p 27(KIP 1)-/- double knockout mice, the corneal epithelium was as thick as in wild-type mice and had many BrdU positive cells. CONCLUSIONS: These results suggest that degradation of p27(KIP1) by Skp 2 is involved in the regulation of proliferation in response to wounding of the corneal epithelium. To examine the involvement of the c-maf gene in the proliferation of the lens cells, eyes of the E13 and E18 stages of wild-type and c-maf-/- mice were analyzed by BrdU incorporation assay, TUNEL assay, and immunocytochemistry using an anti-P 27 (KIP 1) and an anti-P 57 (KIP 2) antibody. In the E 13 and E 18 c-maf mutant lens, BrdU-positive cells were detected at the posterior region of the lens. Cell-cycle inhibitor P 27 (KIP 1) and P 57 (KIP 2) were expressed in the equatorial and posterior region of the lens of both wild-type and c-maf-/- lenses. These results suggest that the expression of c-maf is required for differentiation and cell cycle arrest of lens cells independent of p 27 (KIP 1) and p 57 (KIP 2).

元の言語英語
ページ(範囲)678-686
ページ数9
ジャーナルNippon Ganka Gakkai zasshi
107
発行部数11
出版物ステータス出版済み - 1 1 2003
外部発表Yes

All Science Journal Classification (ASJC) codes

  • Medicine(all)

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    Yoshida, K., Harada, T., Harada, C., Kase, S., Ikeda, H., Sakai, M., Nishi, S., Imaki, J., Nakayama, K., Nagahama, H., Nakayama, K., & Ohno, S. (2003). Proliferative regulation in the cornea and lens. Nippon Ganka Gakkai zasshi, 107(11), 678-686.