Property of IK,n in inner hair cells isolated from guinea-pig cochlea

Takashi Kimitsuki, Noritaka Komune, Teppei Noda, Kazutaka Takaiwa, Mitsuru Ohashi, Shizuo Komune

研究成果: ジャーナルへの寄稿記事

8 引用 (Scopus)

抄録

One of the potassium currents, IK,n, is already activated at the resting potential of the cell and thus determines the membrane potential. KCNQ4 channel has been identified as the molecular correlate of IK,n. In the present study, we measured IK,n in acutely isolated IHCs of guinea-pig cochlea using the whole-cell voltage-clamp techniques, and investigated the properties of the currents. IK,n was 70% activated around the resting potential of -60 mV and deactivated on hyperpolarization. IK,n was blocked by the KCNQ-channel blockers, linopirdine (100 μM) and XE991 (10 μM), but was insensitive to both IK,f blocker, tetraethylammonium (TEA), and IK,s blocker, 4-aminopyridine (4-AP). There was no significant difference in the size of IK,n between the apical and basal turn IHCs.

元の言語英語
ページ(範囲)57-62
ページ数6
ジャーナルHearing Research
261
発行部数1-2
DOI
出版物ステータス出版済み - 3 1 2010
外部発表Yes

Fingerprint

Inner Auditory Hair Cells
Cochlea
Membrane Potentials
Guinea Pigs
linopirdine
4-Aminopyridine
Tetraethylammonium
Patch-Clamp Techniques
Potassium

All Science Journal Classification (ASJC) codes

  • Sensory Systems

これを引用

Property of IK,n in inner hair cells isolated from guinea-pig cochlea. / Kimitsuki, Takashi; Komune, Noritaka; Noda, Teppei; Takaiwa, Kazutaka; Ohashi, Mitsuru; Komune, Shizuo.

:: Hearing Research, 巻 261, 番号 1-2, 01.03.2010, p. 57-62.

研究成果: ジャーナルへの寄稿記事

Kimitsuki, Takashi ; Komune, Noritaka ; Noda, Teppei ; Takaiwa, Kazutaka ; Ohashi, Mitsuru ; Komune, Shizuo. / Property of IK,n in inner hair cells isolated from guinea-pig cochlea. :: Hearing Research. 2010 ; 巻 261, 番号 1-2. pp. 57-62.
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abstract = "One of the potassium currents, IK,n, is already activated at the resting potential of the cell and thus determines the membrane potential. KCNQ4 channel has been identified as the molecular correlate of IK,n. In the present study, we measured IK,n in acutely isolated IHCs of guinea-pig cochlea using the whole-cell voltage-clamp techniques, and investigated the properties of the currents. IK,n was 70{\%} activated around the resting potential of -60 mV and deactivated on hyperpolarization. IK,n was blocked by the KCNQ-channel blockers, linopirdine (100 μM) and XE991 (10 μM), but was insensitive to both IK,f blocker, tetraethylammonium (TEA), and IK,s blocker, 4-aminopyridine (4-AP). There was no significant difference in the size of IK,n between the apical and basal turn IHCs.",
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AU - Kimitsuki, Takashi

AU - Komune, Noritaka

AU - Noda, Teppei

AU - Takaiwa, Kazutaka

AU - Ohashi, Mitsuru

AU - Komune, Shizuo

PY - 2010/3/1

Y1 - 2010/3/1

N2 - One of the potassium currents, IK,n, is already activated at the resting potential of the cell and thus determines the membrane potential. KCNQ4 channel has been identified as the molecular correlate of IK,n. In the present study, we measured IK,n in acutely isolated IHCs of guinea-pig cochlea using the whole-cell voltage-clamp techniques, and investigated the properties of the currents. IK,n was 70% activated around the resting potential of -60 mV and deactivated on hyperpolarization. IK,n was blocked by the KCNQ-channel blockers, linopirdine (100 μM) and XE991 (10 μM), but was insensitive to both IK,f blocker, tetraethylammonium (TEA), and IK,s blocker, 4-aminopyridine (4-AP). There was no significant difference in the size of IK,n between the apical and basal turn IHCs.

AB - One of the potassium currents, IK,n, is already activated at the resting potential of the cell and thus determines the membrane potential. KCNQ4 channel has been identified as the molecular correlate of IK,n. In the present study, we measured IK,n in acutely isolated IHCs of guinea-pig cochlea using the whole-cell voltage-clamp techniques, and investigated the properties of the currents. IK,n was 70% activated around the resting potential of -60 mV and deactivated on hyperpolarization. IK,n was blocked by the KCNQ-channel blockers, linopirdine (100 μM) and XE991 (10 μM), but was insensitive to both IK,f blocker, tetraethylammonium (TEA), and IK,s blocker, 4-aminopyridine (4-AP). There was no significant difference in the size of IK,n between the apical and basal turn IHCs.

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