Propionibacterium acnes acts as an adjuvant in in vitro immunization of human peripheral blood mononuclear cells

Suk Jung Yeon, Shin Ei Matsumoto, Makiko Yamashita, Kosuke Tomimatsu, Kiichiro Teruya, Yoshinori Katakura, Sanetaka Shirahata

研究成果: Contribution to journalArticle査読

7 被引用数 (Scopus)

抄録

We have established an in vitro immunization protocol whereby human peripheral blood mononuclear cells (PBMCs) are initially treated with L-leucyl-L-leucine methyl ester (LLME) and subsequently sensitized with antigen in the presence of interleukin (IL)-2, IL-4, and adjuvant. This protocol resulted in the production of antigen-specific antibodies. PBMCs are potentiated to react with exogenous antigens upon treatment with LLME. We are using this system to investigate the immunomodulatory activity of additives. In the present study, we aimed to evaluate the immunomodulatory activity of Propionibacterium acnes (P. acnes), which is known to exhibit various immunomodulatory effects in murine models, using this in vitro immunization protocol. P. acnes was found to augment the production of antigen-specific antibodies by PBMC, possibly through increased production of inflammatory cytokines and/or increased T-B cell interaction. P. acnes hence appears to act as an adjuvant in the antibody response in in vitro immunization.

本文言語英語
ページ(範囲)1963-1969
ページ数7
ジャーナルBioscience, Biotechnology and Biochemistry
71
8
DOI
出版ステータス出版済み - 11 21 2007

All Science Journal Classification (ASJC) codes

  • Bioengineering
  • Biotechnology
  • Biochemistry
  • Biochemistry, Genetics and Molecular Biology(all)
  • Chemistry (miscellaneous)
  • Applied Microbiology and Biotechnology
  • Food Science

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