Prospective isolation of human clonogenic common myeloid progenitors

Markus G. Manz, Toshihiro Miyamoto, Koichi Akashi, Irving L. Weissman

研究成果: ジャーナルへの寄稿学術誌査読

388 被引用数 (Scopus)

抄録

The hierarchical development from hematopoietic stem cells to mature cells of the hematolymphoid system involves progressive loss of self-renewal capacity, proliferation ability, and lineage potentials. Here we show the prospective isolation of early developmental intermediates, the human clonogenic common myeloid progenitors and their downstream progeny, the granulocyte/macrophage and megakaryocyte/erythrocyte progenitors. All three populations reside in the lineage-negative (lin-) CD34+CD38+ fraction of adult bone marrow as well as in cord blood. They are distinguishable by the expression of the IL-3Rα chain, the receptor of an early-acting hematopoietic cytokine, and CD45RA, an isoform of a phosphotyrosine phosphatase involved in negative regulation of cytokine signaling. Multipotent progenitors, early lymphoid progenitors, and the here-defined myeloid progenitors express distinct profiles of hematopoiesis-affiliated genes. The isolation of highly purified hematopoietic intermediates provides tools to better understand developmental programs underlying normal and leukemic hematopoiesis.

本文言語英語
ページ(範囲)11872-11877
ページ数6
ジャーナルProceedings of the National Academy of Sciences of the United States of America
99
18
DOI
出版ステータス出版済み - 9月 3 2002

!!!All Science Journal Classification (ASJC) codes

  • 一般

フィンガープリント

「Prospective isolation of human clonogenic common myeloid progenitors」の研究トピックを掘り下げます。これらがまとまってユニークなフィンガープリントを構成します。

引用スタイル