Prostaglandin E2 (PGE2) has been shown to produce fever by acting on EP3 receptors within the preoptic area of the brain. However, there is little information about the molecular events downstream of EP3 activation in preoptic neurons. As a first step toward this issue, we examined PGE2-induced gene expression changes at single-cell resolution in preoptic neurons expressing EP3. Brain sections of the preoptic area from PGE2-or saline-injected rats were stained with an anti-EP3 antibody, and the cell bodies of EP3-positive neurons were dissected and subjected to RNA amplification procedures. Microarray analysis of the amplified products demonstrated the possibility that gene expression of γ-aminobutyric acid type A (GABAA) receptor subunits is decreased upon PGE2 injection. Indeed, we found that most EP3-positive neurons in the mouse preoptic area are positive for the α2 or γ2 GABAA receptor subunit. Moreover, PGE2 decreased the preoptic gene expression of these GABAA subunits via an EP3-dependent and pertussis toxin-sensitive pathway. PGE2 also attenuated the preoptic protein expression of the α2 subunit in wild-type but not in EP3-deficient mice. These results indicate that PGE2-EP3 signaling elicits G i/oactivation in preoptic thermocenter neurons, and we propose the possibility that a rapid decrease in preoptic GABAA expression may be involved in PGE2-induced fever.
All Science Journal Classification (ASJC) codes
- Molecular Biology
- Cell Biology