Protection against Campylobacter jejuni Infection in Suckling Mice by Anti-Flagellar Antibody

Yuji Ueki, Akiko Umeda, Shuji Fujimoto, Kazunobu Amako, Masao Mitsuyama

研究成果: Contribution to journalArticle査読

19 被引用数 (Scopus)

抄録

We obtained two monoclonal antibodies of IgM class and IgA class of immunoglobulin prepared from mouse spleen cells immunized with crude flagellar preparation, and a polyclonal antibody raised against purified flagellin monomer of Campylobacter jejuni in a rabbit. The specificity of the reaction of these antibodies for flagellar filament was confirmed by Western blotting and by immunoelectron microscopy. These antibodies caused agglutination of the bacteria and inhibited the motility of the bacteria. When a strain of C. jejuni was treated with IgM class monoclonal antibody before being inoculated into suckling mice, it reduced colonization of the intestinal tract by this bacteria. Inhibition of the colonization by IgA class monoclonal antibody was less effective than that of IgM class, and the polyclonal antibody consisting mostly of IgG class immunoglobulin was without effect.

本文言語英語
ページ(範囲)1161-1171
ページ数11
ジャーナルMICROBIOLOGY and IMMUNOLOGY
31
12
DOI
出版ステータス出版済み - 1987

All Science Journal Classification (ASJC) codes

  • 微生物学
  • 免疫学
  • ウイルス学

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