Protein aggregates are transported to vacuoles by a macroautophagic mechanism in nutrient-starved plant cells

Kiminori Toyooka, Yuji Moriyasu, Yumi Goto, Masaki Takeuchi, Hiroo Fukuda, Ken Matsuoka

研究成果: ジャーナルへの寄稿学術誌査読

88 被引用数 (Scopus)

抄録

When a fusion protein of cytochrome b5 (Cyt b5) and the red fluorescent protein (RFP) are expressed in tobacco BY-2 cells, the expressed protein forms intracellular aggregates that emit red fluorescence. When such cells are grown to the stationary phase or incubated in nutrient limited medium, RFP fluorescence can be detected in the vacuolar lumen. We investigated this transport mechanism using a limited-nitrogen model. E-64 and 3-methyladenine, which inhibit autophagic processes, blocked the transport of the RFP signal to the vacuole. We next traced the autophagic process in tobacco cells using YFP fused with the tobacco Atg8 homologue (YFP-NtAtg8) and analyzed the contribution of autophagy to the vacuolar transport of the aggregates. Under limited-nitrogen conditions, the aggregates were degraded in preference to other organelles, and the autophagosomes colocalized with the aggregates at a higher frequency than with mitochondria. This is the first demonstration that selective macroautophagic degradation can occur in plant cells.

本文言語英語
ページ(範囲)96-106
ページ数11
ジャーナルAutophagy
2
2
DOI
出版ステータス出版済み - 1月 1 2006
外部発表はい

!!!All Science Journal Classification (ASJC) codes

  • 分子生物学
  • 細胞生物学

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