Protein coverage on polymer nanolayers leading to mesenchymal stem cell patterning

Jungmok You, Akihito Yoshida, June Seok Heo, Han Soo Kim, Hyun Ok Kim, Kaoru Tamada, Eunkyoung Kim

研究成果: Contribution to journalArticle査読

12 被引用数 (Scopus)

抄録

Interactions of gelatin and albumin with a photo-reactive diphenylamino-s-triazine bridged p-phenylene vinylene polymer (DTOPV) were examined by using surface plasmon resonance (SPR) spectroscopy to explore the effect of the polymer structure on protein coverage of DTOPV nanofilms. The SPR data revealed a significant increase of gelatin adsorption on UV-DTOPV nanofilms, while the adsorption of albumin was decreased by UV exposure in the time frame of the experiment. We also found that the selective adsorption of these proteins was highly dependent on the protein concentration; the highest selectivity of protein adsorption was obtained at the lowest concentration (3.5 μg ml -1), while no selective adsorption was confirmed at high concentrations (350 and 1000 μg ml -1). The selective attachment of mesenchymal stem cells (MSCs) was directly correlated with the selective adsorption of these proteins onto DTOPV nanofilms. The MSCs attachment onto UV-DTOPV films was promoted with only small mass coverage of gelatin, which led to MSC patterning onto the patterned DTOPV nanofilms successfully. The role of cell adhesion proteins that we found in this study will be a clue to elucidate the complex response of biomolecules on functional polymer nanolayers, and contribute to build up biocompatible surfaces on various advanced materials for the sake of cell engineering and medical implants. This journal is

本文言語英語
ページ(範囲)17625-17632
ページ数8
ジャーナルPhysical Chemistry Chemical Physics
13
39
DOI
出版ステータス出版済み - 10 21 2011
外部発表はい

All Science Journal Classification (ASJC) codes

  • Physics and Astronomy(all)
  • Physical and Theoretical Chemistry

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