抄録
Transglutaminase (TG) plays important and diverse roles in mammals, such as blood coagulation and formation of the skin barrier, by catalyzing protein crosslinking. In invertebrates, TG is known to be involved in immobilization of invading pathogens at sites of injury. Here we demonstrate that Drosophila TG is an important enzyme for cuticle morphogenesis. Although TG activity was undetectable before the second instar larval stage, it dramatically increased in the third instar larval stage. RNA interference (RNAi) of the TG gene caused a pupal semi-lethal phenotype and abnormal morphology. Furthermore, TG-RNAi flies showed a significantly shorter life span than their counterparts, and approximately 90% of flies died within 30 days after eclosion. Stage-specific TG-RNAi before the third instar larval stage resulted in cuticle abnormality, but the TG-RNAi after the late pupal stage did not, indicating that TG plays a key role at or before the early pupal stage. Immediately following eclosion, acid-extractable protein from wild-type wings was nearly all converted to non-extractable protein due to wing maturation, whereas several proteins remained acid-extractable in the mature wings of TG-RNAi flies. We identified four proteins-two cuticular chitin-binding proteins, larval serum protein 2, and a putative C-type lectin-as TG substrates. RNAi of their corresponding genes caused a lethal phenotype or cuticle abnormality. Our results indicate that TG-dependent protein crosslinking in Drosophila plays a key role in cuticle morphogenesis and sclerotization.
| 元の言語 | 英語 |
|---|---|
| 記事番号 | e13477 |
| ジャーナル | PloS one |
| 巻 | 5 |
| 発行部数 | 10 |
| DOI | |
| 出版物ステータス | 出版済み - 11 17 2010 |
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All Science Journal Classification (ASJC) codes
- Biochemistry, Genetics and Molecular Biology(all)
- Agricultural and Biological Sciences(all)
- General
これを引用
Protein crosslinking by transglutaminase controls cuticle morphogenesis in Drosophila. / Shibata, Toshio; Ariki, Shigeru; Shinzawa, Naoaki; Miyaji, Ryuta; Suyama, Haruka; Sako, Miyuki; Inomata, Nobuyuki; Koshiba, Takumi; Kanuka, Hirotaka; Kawabata, Shun Ichiro.
：: PloS one, 巻 5, 番号 10, e13477, 17.11.2010.研究成果: ジャーナルへの寄稿 › 記事
}
TY - JOUR
T1 - Protein crosslinking by transglutaminase controls cuticle morphogenesis in Drosophila
AU - Shibata, Toshio
AU - Ariki, Shigeru
AU - Shinzawa, Naoaki
AU - Miyaji, Ryuta
AU - Suyama, Haruka
AU - Sako, Miyuki
AU - Inomata, Nobuyuki
AU - Koshiba, Takumi
AU - Kanuka, Hirotaka
AU - Kawabata, Shun Ichiro
PY - 2010/11/17
Y1 - 2010/11/17
N2 - Transglutaminase (TG) plays important and diverse roles in mammals, such as blood coagulation and formation of the skin barrier, by catalyzing protein crosslinking. In invertebrates, TG is known to be involved in immobilization of invading pathogens at sites of injury. Here we demonstrate that Drosophila TG is an important enzyme for cuticle morphogenesis. Although TG activity was undetectable before the second instar larval stage, it dramatically increased in the third instar larval stage. RNA interference (RNAi) of the TG gene caused a pupal semi-lethal phenotype and abnormal morphology. Furthermore, TG-RNAi flies showed a significantly shorter life span than their counterparts, and approximately 90% of flies died within 30 days after eclosion. Stage-specific TG-RNAi before the third instar larval stage resulted in cuticle abnormality, but the TG-RNAi after the late pupal stage did not, indicating that TG plays a key role at or before the early pupal stage. Immediately following eclosion, acid-extractable protein from wild-type wings was nearly all converted to non-extractable protein due to wing maturation, whereas several proteins remained acid-extractable in the mature wings of TG-RNAi flies. We identified four proteins-two cuticular chitin-binding proteins, larval serum protein 2, and a putative C-type lectin-as TG substrates. RNAi of their corresponding genes caused a lethal phenotype or cuticle abnormality. Our results indicate that TG-dependent protein crosslinking in Drosophila plays a key role in cuticle morphogenesis and sclerotization.
AB - Transglutaminase (TG) plays important and diverse roles in mammals, such as blood coagulation and formation of the skin barrier, by catalyzing protein crosslinking. In invertebrates, TG is known to be involved in immobilization of invading pathogens at sites of injury. Here we demonstrate that Drosophila TG is an important enzyme for cuticle morphogenesis. Although TG activity was undetectable before the second instar larval stage, it dramatically increased in the third instar larval stage. RNA interference (RNAi) of the TG gene caused a pupal semi-lethal phenotype and abnormal morphology. Furthermore, TG-RNAi flies showed a significantly shorter life span than their counterparts, and approximately 90% of flies died within 30 days after eclosion. Stage-specific TG-RNAi before the third instar larval stage resulted in cuticle abnormality, but the TG-RNAi after the late pupal stage did not, indicating that TG plays a key role at or before the early pupal stage. Immediately following eclosion, acid-extractable protein from wild-type wings was nearly all converted to non-extractable protein due to wing maturation, whereas several proteins remained acid-extractable in the mature wings of TG-RNAi flies. We identified four proteins-two cuticular chitin-binding proteins, larval serum protein 2, and a putative C-type lectin-as TG substrates. RNAi of their corresponding genes caused a lethal phenotype or cuticle abnormality. Our results indicate that TG-dependent protein crosslinking in Drosophila plays a key role in cuticle morphogenesis and sclerotization.
UR - http://www.scopus.com/inward/record.url?scp=78149426519&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=78149426519&partnerID=8YFLogxK
U2 - 10.1371/journal.pone.0013477
DO - 10.1371/journal.pone.0013477
M3 - Article
C2 - 20976106
AN - SCOPUS:78149426519
VL - 5
JO - PLoS One
JF - PLoS One
SN - 1932-6203
IS - 10
M1 - e13477
ER -