Protein Crystallization in Microdroplets with the Aid of Electrically Induced Microbubbles

Akiho Hirao, Naotomo Tottori, Maasa Yokomori, Miho Tagawa, Shigeo S. Sugano, Shinya Sakuma, Yoko Yamanishi

研究成果: Chapter in Book/Report/Conference proceedingConference contribution

抄録

We present a novel concept of microfluidic system that enable promoting the protein crystal nucleation, monitoring the protein crystallization state, and analysis the crystal structure in a sequential process. This proposed system comprised of four main functional parts: (i) the protein crystal nucleation using an electrically induced microbubble injector, (ii) droplets generation by microchannel, (iii) monitoring of a crystal morphology in droplets to optimize their incubation time, (iv) sorting of droplets containing produced crystals suitable for X-ray crystallography and analyzing the protein structure. As the first step of this research, in this study, we developed a critical functional part of the concept that enable control of the protein crystallization and could confirm the mean number of protein crystal in droplets, which was treated by electrically induced bubble, increased approximately 1.5 times as compared with that of protein crystal in untreated droplets.

本文言語英語
ホスト出版物のタイトル34th IEEE International Conference on Micro Electro Mechanical Systems, MEMS 2021
出版社Institute of Electrical and Electronics Engineers Inc.
ページ168-171
ページ数4
ISBN(電子版)9781665419123
DOI
出版ステータス出版済み - 1 25 2021
イベント34th IEEE International Conference on Micro Electro Mechanical Systems, MEMS 2021 - Virtual, Gainesville, 米国
継続期間: 1 25 20211 29 2021

出版物シリーズ

名前Proceedings of the IEEE International Conference on Micro Electro Mechanical Systems (MEMS)
2021-January
ISSN(印刷版)1084-6999

会議

会議34th IEEE International Conference on Micro Electro Mechanical Systems, MEMS 2021
国/地域米国
CityVirtual, Gainesville
Period1/25/211/29/21

All Science Journal Classification (ASJC) codes

  • 電子材料、光学材料、および磁性材料
  • 凝縮系物理学
  • 機械工学
  • 電子工学および電気工学

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