Protein engineering and the study of muscle contraction in Drosophila flight muscles

J. Sparrow, Douglas Robert Drummond, M. Peckham, E. Hennessey, D. White

研究成果: ジャーナルへの寄稿記事

16 引用 (Scopus)

抄録

We describe an experimental approach to the use of genetics to study muscle contraction in Drosophila melanogaster. Mutations induced by in vitro mutagenesis are inserted into the genome of flies using P-element mediated transformation, permitting the effects of the mutant genes to be studied in vivo in the indirect flight muscles (IFMs). Details of how mechanical experiments can be performed on skinned IFMs, despite their small size, are provided. The effects of two in vitro actin mutations, G368E and E316K, are described. The problems of performing biochemical and biophysical experiments on the IFMs and their myofibrillar proteins are described, together with indications as to how these may be overcome.

元の言語英語
ページ(範囲)73-78
ページ数6
ジャーナルJournal of Cell Science
98
発行部数SUPPL. 14
出版物ステータス出版済み - 5 14 1991
外部発表Yes

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Protein Engineering
Muscle Contraction
Drosophila
Muscles
Mutation
Drosophila melanogaster
Mutagenesis
Diptera
Actins
Genome
Genes
Proteins
In Vitro Techniques

All Science Journal Classification (ASJC) codes

  • Cell Biology

これを引用

Sparrow, J., Drummond, D. R., Peckham, M., Hennessey, E., & White, D. (1991). Protein engineering and the study of muscle contraction in Drosophila flight muscles. Journal of Cell Science, 98(SUPPL. 14), 73-78.

Protein engineering and the study of muscle contraction in Drosophila flight muscles. / Sparrow, J.; Drummond, Douglas Robert; Peckham, M.; Hennessey, E.; White, D.

:: Journal of Cell Science, 巻 98, 番号 SUPPL. 14, 14.05.1991, p. 73-78.

研究成果: ジャーナルへの寄稿記事

Sparrow, J, Drummond, DR, Peckham, M, Hennessey, E & White, D 1991, 'Protein engineering and the study of muscle contraction in Drosophila flight muscles', Journal of Cell Science, 巻. 98, 番号 SUPPL. 14, pp. 73-78.
Sparrow, J. ; Drummond, Douglas Robert ; Peckham, M. ; Hennessey, E. ; White, D. / Protein engineering and the study of muscle contraction in Drosophila flight muscles. :: Journal of Cell Science. 1991 ; 巻 98, 番号 SUPPL. 14. pp. 73-78.
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