Protein engineering of novel proteinase inhibitors and their effects on the growth of Spodoptera exigua larvae

Hideko Inanaga, Daiki Kobayasi, Yoshiaki Kouzuma, Chisa Yasunaga-Aoki, Kazuhiro Iiyama, Makoto Kimura

研究成果: ジャーナルへの寄稿記事

21 引用 (Scopus)

抄録

Novel types of proteinase inhibitors with multi-inhibitory activities were generated by replacement of phytocystatin domains in sunflower multi-cystatin (SMC) by the serine proteinase inhibitor BGIT from bitter gourd seeds. Two chimeric inhibitors SMC-T3 and SMC-T23, in which the third domain in SMC and the second and third domains in SMC were replaced by BGIT, acquired trypsin inhibitory activity (Ki: 1.46 × 10-7 M and 1.75 × 10-7 M), retaining inhibitory activity toward papain (Ki: 4.5 × 10-8 M and 1.52 × 10-7 M), respectively. We compared the chimeric inhibitors and the recombinant SMC (r-SMC) in relation to their effects on the growth of larval Spodoptera exigua. When the second instar larvae were reared on a diet containing rSMC, SMC-T3, or SMC-T23 for ten days, a significant reduction in weight gain was observed. Mean weights for rSMC, SMC-T3, and SMC-T23 were 43 mg, 32 mg, and 43 mg, respectively, as compared with that (60 mg) for the absence of the inhibitor. In contrast, BGIT had little effect on the growth of the S. exigua larvae. This result indicated that the chimeric inhibitor SMC-T3 with two phytocystatin domains and one serine proteinase inhibitor domain is an efficient inhibitor of proteinases in the S. exigua larvae. Therefore, this novel type of proteinase inhibitor with multi-inhibitory activities may represent a promising protein for successful application to a transgenic plant with insect resistance.

元の言語英語
ページ(範囲)2259-2264
ページ数6
ジャーナルBioscience, Biotechnology and Biochemistry
65
発行部数10
DOI
出版物ステータス出版済み - 10 1 2001

Fingerprint

Cystatins
Protein Engineering
Spodoptera
Helianthus
Larva
Peptide Hydrolases
Growth
Proteins
Serine Proteinase Inhibitors
Momordica charantia
Papain
Genetically Modified Plants
Nutrition
Trypsin
Weight Gain
Insects
Seed
Seeds

All Science Journal Classification (ASJC) codes

  • Biotechnology
  • Analytical Chemistry
  • Biochemistry
  • Applied Microbiology and Biotechnology
  • Molecular Biology
  • Organic Chemistry

これを引用

Protein engineering of novel proteinase inhibitors and their effects on the growth of Spodoptera exigua larvae. / Inanaga, Hideko; Kobayasi, Daiki; Kouzuma, Yoshiaki; Yasunaga-Aoki, Chisa; Iiyama, Kazuhiro; Kimura, Makoto.

:: Bioscience, Biotechnology and Biochemistry, 巻 65, 番号 10, 01.10.2001, p. 2259-2264.

研究成果: ジャーナルへの寄稿記事

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