Protein extraction was performed by a calixarene carboxylic acid derivative in isooctane. Although the calixarene has a drawback in that it is not solubilized in aliphatic organic solvents, addition of alcohols overcomes the problem. The effect of alcohols on the extraction of cytochrome c (Cyt-c) was investigated with calixarene in isooctane. Normal octanol provided the best result for the Cyt-c extraction. The pH value in the aqueous phase was one of the key operational factors in the liquid-liquid extraction process. It was confirmed that the extracted proteins maintain their biological activity during the extraction operation.
|ジャーナル||Solvent Extraction Research and Development|
|出版ステータス||出版済み - 12月 1 2003|
!!!All Science Journal Classification (ASJC) codes
- 化学 (全般)