Protein extraction by calix[6]arene in an aliphatic organic solvent

Kojiro Shimojo, Junji Watanabe, Tatsuya Oshima, Masahiro Goto

    研究成果: ジャーナルへの寄稿学術誌査読

    18 被引用数 (Scopus)

    抄録

    Protein extraction was performed by a calix[6]arene carboxylic acid derivative in isooctane. Although the calixarene has a drawback in that it is not solubilized in aliphatic organic solvents, addition of alcohols overcomes the problem. The effect of alcohols on the extraction of cytochrome c (Cyt-c) was investigated with calix[6]arene in isooctane. Normal octanol provided the best result for the Cyt-c extraction. The pH value in the aqueous phase was one of the key operational factors in the liquid-liquid extraction process. It was confirmed that the extracted proteins maintain their biological activity during the extraction operation.

    本文言語英語
    ページ(範囲)185-189
    ページ数5
    ジャーナルSolvent Extraction Research and Development
    10
    出版ステータス出版済み - 12月 1 2003

    !!!All Science Journal Classification (ASJC) codes

    • 化学 (全般)
    • 化学工学(全般)

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