Protein-immobilized Electrode for Rapid and Convenient Sensing of Thyroid Hormone Receptor-ligand Interaction

Masaharu Murata, Kentaro Yano, Shinichiro Kuroki, Tatsuo Suzutani, Yoshiki Katayama

研究成果: ジャーナルへの寄稿記事

8 引用 (Scopus)

抄録

The recombinant human thyroid hormone receptor (TR) was expressed as an in-frame fusion with ten consecutive histidine residues using a bacterial system; then the receptor was immobilized on an Au-electrode with Ni(II)-mediated chemisorption using the histidine tag and thiol-modified nitrilotriacetic acid. The receptor-modified electrode could rapidly detect ligand binding to hTR without any separation procedures, and showed a good response in a concentration-dependent manner. The sensitivity of this biosensor based on ligand-receptor interactions was comparable to those of conventional competitive ligand binding assays using radio-labeled ligands. Our results strongly suggest that our new biosensor can be applied to the identification of new ligands for hTR.

元の言語英語
ページ(範囲)1569-1573
ページ数5
ジャーナルanalytical sciences
19
発行部数12
DOI
出版物ステータス出版済み - 1 1 2003

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Immobilized Proteins
Thyroid Hormone Receptors
Ligands
Electrodes
Histidine
Biosensors
Nitrilotriacetic Acid
Chemisorption
Sulfhydryl Compounds
Assays
Fusion reactions

All Science Journal Classification (ASJC) codes

  • Analytical Chemistry

これを引用

Protein-immobilized Electrode for Rapid and Convenient Sensing of Thyroid Hormone Receptor-ligand Interaction. / Murata, Masaharu; Yano, Kentaro; Kuroki, Shinichiro; Suzutani, Tatsuo; Katayama, Yoshiki.

:: analytical sciences, 巻 19, 番号 12, 01.01.2003, p. 1569-1573.

研究成果: ジャーナルへの寄稿記事

Murata, Masaharu ; Yano, Kentaro ; Kuroki, Shinichiro ; Suzutani, Tatsuo ; Katayama, Yoshiki. / Protein-immobilized Electrode for Rapid and Convenient Sensing of Thyroid Hormone Receptor-ligand Interaction. :: analytical sciences. 2003 ; 巻 19, 番号 12. pp. 1569-1573.
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