Protein-immobilized Electrode for Rapid and Convenient Sensing of Thyroid Hormone Receptor-ligand Interaction

Masaharu Murata, Kentaro Yano, Shinichiro Kuroki, Tatsuo Suzutani, Yoshiki Katayama

研究成果: ジャーナルへの寄稿学術誌査読

8 被引用数 (Scopus)

抄録

The recombinant human thyroid hormone receptor (TR) was expressed as an in-frame fusion with ten consecutive histidine residues using a bacterial system; then the receptor was immobilized on an Au-electrode with Ni(II)-mediated chemisorption using the histidine tag and thiol-modified nitrilotriacetic acid. The receptor-modified electrode could rapidly detect ligand binding to hTR without any separation procedures, and showed a good response in a concentration-dependent manner. The sensitivity of this biosensor based on ligand-receptor interactions was comparable to those of conventional competitive ligand binding assays using radio-labeled ligands. Our results strongly suggest that our new biosensor can be applied to the identification of new ligands for hTR.

本文言語英語
ページ(範囲)1569-1573
ページ数5
ジャーナルanalytical sciences
19
12
DOI
出版ステータス出版済み - 12月 2003

!!!All Science Journal Classification (ASJC) codes

  • 分析化学

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