Protein kinase Cα-responsive polymeric carrier: Its application for gene delivery into human cancers

Tetsuro Tomiyama, Jeong Hun Kang, Riki Toita, Takuro Niidome, Yoshiki Katayama

研究成果: ジャーナルへの寄稿記事

17 引用 (Scopus)

抄録

For cancer-targeting gene delivery, we applied a protein kinase C (PKC)α-responsive polymeric carrier to human cancers (U-87 MG [human glioblastoma-astrocytoma, epithelial-like cell line]and A549 [human lung adenocarcinoma epithelial cell line]). Two polymers, one a PKCα-responsive polymer (PPC[S]) containing the phosphorylation site serine, and the other a negative control polymer (PPC[A]), in which the serine was substituted with alanine, were synthesized. No cytotoxicity of the polymer was identified. When the complexes were transfected into cancer cells or tissues in which PKCα was hyper-activated, the luciferase expression from the PPC(S)/plasmid (pDNA) complex was higher than that from the PPC(A)/pDNA complex. These results show that the phosphorylation of complex by PKCα in cancer cells leads to high gene expression and that our system can be used as a human cancer cell-targeting gene delivery system.

元の言語英語
ページ(範囲)1532-1536
ページ数5
ジャーナルCancer Science
100
発行部数8
DOI
出版物ステータス出版済み - 7 23 2009

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Protein Kinase C
Polymers
Serine
Genes
Neoplasms
Epithelial Cells
Phosphorylation
Cell Line
Gene Transfer Techniques
Neoplasm Genes
Astrocytoma
Glioblastoma
Luciferases
Alanine
Plasmids
Gene Expression

All Science Journal Classification (ASJC) codes

  • Oncology
  • Cancer Research

これを引用

Protein kinase Cα-responsive polymeric carrier : Its application for gene delivery into human cancers. / Tomiyama, Tetsuro; Kang, Jeong Hun; Toita, Riki; Niidome, Takuro; Katayama, Yoshiki.

:: Cancer Science, 巻 100, 番号 8, 23.07.2009, p. 1532-1536.

研究成果: ジャーナルへの寄稿記事

Tomiyama, Tetsuro ; Kang, Jeong Hun ; Toita, Riki ; Niidome, Takuro ; Katayama, Yoshiki. / Protein kinase Cα-responsive polymeric carrier : Its application for gene delivery into human cancers. :: Cancer Science. 2009 ; 巻 100, 番号 8. pp. 1532-1536.
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