Protein kinase C regulates the tonic but not the phasic component of contraction in guinea-pig ileum

T. Sasaguri, S. P. Watson

研究成果: ジャーナルへの寄稿記事

19 引用 (Scopus)

抄録

We have investigated the effect of phorbol esters and the down-regulation of protein kinase C on contraction of guinea-pig ileum longitudinal smooth muscle to carbachol and high K+. Phorbol 12,13-dibutyrate (PDBu) enhanced the phasic component and inhibited or enhanced, respectively, the tonic component of contraction to carbachol and high K+. In contrast, 4α-phorbol, which does not activate protein kinase C, had no effect on these responses. Exposure to phorbol 12-myristate 13-acetate (PMA; 1 μM) for up to 8 h induced a time-dependent loss of [3H]-PDBu binding sites, consistent with the down-regulation of protein kinase C by this treatment. The phasic component of contraction to carbachol or high K+ was unaffected following the down-regulation of protein kinase C. The tonic component of contraction to carbachol was markedly enhanced by this treatment while that to high K+ was partially suppressed. These data suggest that although the activation of protein kinase C can lead to potentiation of the phasic component of contraction to carbachol or high K+, this appears to have little physiological significance since the response is not altered in tissues in which protein kinase C has been down-regulated. On the other hand, protein kinase C may limit the tonic contraction to carbachol but potentiate that to high K+.

元の言語英語
ページ(範囲)791-798
ページ数8
ジャーナルBritish Journal of Pharmacology
98
発行部数3
DOI
出版物ステータス出版済み - 1 1 1989

Fingerprint

Ileum
Carbachol
Protein Kinase C
Guinea Pigs
Phorbol 12,13-Dibutyrate
Down-Regulation
Phorbol Esters
Smooth Muscle
Acetates
Binding Sites

All Science Journal Classification (ASJC) codes

  • Pharmacology

これを引用

Protein kinase C regulates the tonic but not the phasic component of contraction in guinea-pig ileum. / Sasaguri, T.; Watson, S. P.

:: British Journal of Pharmacology, 巻 98, 番号 3, 01.01.1989, p. 791-798.

研究成果: ジャーナルへの寄稿記事

@article{ded51984f2eb4f32a3984beb2ffce615,
title = "Protein kinase C regulates the tonic but not the phasic component of contraction in guinea-pig ileum",
abstract = "We have investigated the effect of phorbol esters and the down-regulation of protein kinase C on contraction of guinea-pig ileum longitudinal smooth muscle to carbachol and high K+. Phorbol 12,13-dibutyrate (PDBu) enhanced the phasic component and inhibited or enhanced, respectively, the tonic component of contraction to carbachol and high K+. In contrast, 4α-phorbol, which does not activate protein kinase C, had no effect on these responses. Exposure to phorbol 12-myristate 13-acetate (PMA; 1 μM) for up to 8 h induced a time-dependent loss of [3H]-PDBu binding sites, consistent with the down-regulation of protein kinase C by this treatment. The phasic component of contraction to carbachol or high K+ was unaffected following the down-regulation of protein kinase C. The tonic component of contraction to carbachol was markedly enhanced by this treatment while that to high K+ was partially suppressed. These data suggest that although the activation of protein kinase C can lead to potentiation of the phasic component of contraction to carbachol or high K+, this appears to have little physiological significance since the response is not altered in tissues in which protein kinase C has been down-regulated. On the other hand, protein kinase C may limit the tonic contraction to carbachol but potentiate that to high K+.",
author = "T. Sasaguri and Watson, {S. P.}",
year = "1989",
month = "1",
day = "1",
doi = "10.1111/j.1476-5381.1989.tb14607.x",
language = "English",
volume = "98",
pages = "791--798",
journal = "British Journal of Pharmacology",
issn = "0007-1188",
publisher = "Wiley-Blackwell",
number = "3",

}

TY - JOUR

T1 - Protein kinase C regulates the tonic but not the phasic component of contraction in guinea-pig ileum

AU - Sasaguri, T.

AU - Watson, S. P.

PY - 1989/1/1

Y1 - 1989/1/1

N2 - We have investigated the effect of phorbol esters and the down-regulation of protein kinase C on contraction of guinea-pig ileum longitudinal smooth muscle to carbachol and high K+. Phorbol 12,13-dibutyrate (PDBu) enhanced the phasic component and inhibited or enhanced, respectively, the tonic component of contraction to carbachol and high K+. In contrast, 4α-phorbol, which does not activate protein kinase C, had no effect on these responses. Exposure to phorbol 12-myristate 13-acetate (PMA; 1 μM) for up to 8 h induced a time-dependent loss of [3H]-PDBu binding sites, consistent with the down-regulation of protein kinase C by this treatment. The phasic component of contraction to carbachol or high K+ was unaffected following the down-regulation of protein kinase C. The tonic component of contraction to carbachol was markedly enhanced by this treatment while that to high K+ was partially suppressed. These data suggest that although the activation of protein kinase C can lead to potentiation of the phasic component of contraction to carbachol or high K+, this appears to have little physiological significance since the response is not altered in tissues in which protein kinase C has been down-regulated. On the other hand, protein kinase C may limit the tonic contraction to carbachol but potentiate that to high K+.

AB - We have investigated the effect of phorbol esters and the down-regulation of protein kinase C on contraction of guinea-pig ileum longitudinal smooth muscle to carbachol and high K+. Phorbol 12,13-dibutyrate (PDBu) enhanced the phasic component and inhibited or enhanced, respectively, the tonic component of contraction to carbachol and high K+. In contrast, 4α-phorbol, which does not activate protein kinase C, had no effect on these responses. Exposure to phorbol 12-myristate 13-acetate (PMA; 1 μM) for up to 8 h induced a time-dependent loss of [3H]-PDBu binding sites, consistent with the down-regulation of protein kinase C by this treatment. The phasic component of contraction to carbachol or high K+ was unaffected following the down-regulation of protein kinase C. The tonic component of contraction to carbachol was markedly enhanced by this treatment while that to high K+ was partially suppressed. These data suggest that although the activation of protein kinase C can lead to potentiation of the phasic component of contraction to carbachol or high K+, this appears to have little physiological significance since the response is not altered in tissues in which protein kinase C has been down-regulated. On the other hand, protein kinase C may limit the tonic contraction to carbachol but potentiate that to high K+.

UR - http://www.scopus.com/inward/record.url?scp=0024460735&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0024460735&partnerID=8YFLogxK

U2 - 10.1111/j.1476-5381.1989.tb14607.x

DO - 10.1111/j.1476-5381.1989.tb14607.x

M3 - Article

C2 - 2590770

AN - SCOPUS:0024460735

VL - 98

SP - 791

EP - 798

JO - British Journal of Pharmacology

JF - British Journal of Pharmacology

SN - 0007-1188

IS - 3

ER -