Protein p67: A calcium-binding protein localized at the sarcolemma of secretory atrial myocytes

Hiroshi Iida, H. Nishitani, Y. Shibata

研究成果: ジャーナルへの寄稿記事

15 引用 (Scopus)

抄録

Bovine heart 67-kd protein (p67) was coisolated with calpactin I complex by cycles of Ca2+-dependent precipitation followed by solubilization with EGTA-containing buffer. Using affinity-purified anti-p67 antibody and anti-p36 (36-kd subunit of calpactin I) antibody, we examined the localization of the two proteins in secretory atrial myocytes and other endocrine tissues of adult rats. Immunofluorescence microscopy showed that p67 was expressed both in the atrial myocytes in situ and in cultured atrial myocytes in which we failed to detect p36 and that p67 appeared to be closely associated with the cell surface. We also found that p67 was colocalized with p36 in the thyroid follicle epithelium and zona reticularis of the adrenal gland. On the other hand, neither p67 nor p36 was detectable in pancreas islet cells. Immunoelectron microscopy revealed that p67 was localized at the sarcolemma in the atrial myocytes in situ. The p67, which was shown to be a globular molecule with a diameter of 18-25 nm by a low-angle rotary shadowing method, bound radioactive Ca2+ on a nitrocellulose membrane. The results suggest that Ca2+-binding proteins expressed in endocrine cells seem to vary from tissue to tissue and that p67 may function in Ca2+-mediated events at the plasma membrane of secretory atrial myocytes and some types of endocrine cells expressing this protein.

元の言語英語
ページ(範囲)370-381
ページ数12
ジャーナルCirculation research
70
発行部数2
DOI
出版物ステータス出版済み - 1 1 1992

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Sarcolemma
Calcium-Binding Proteins
Muscle Cells
Annexin A2
Endocrine Cells
Proteins
Zona Reticularis
Collodion
Immunoelectron Microscopy
Egtazic Acid
Adrenal Glands
Islets of Langerhans
Fluorescence Microscopy
Pancreas
Anti-Idiotypic Antibodies
Carrier Proteins
Buffers
Thyroid Gland
Epithelium
Cell Membrane

All Science Journal Classification (ASJC) codes

  • Physiology
  • Cardiology and Cardiovascular Medicine

これを引用

Protein p67 : A calcium-binding protein localized at the sarcolemma of secretory atrial myocytes. / Iida, Hiroshi; Nishitani, H.; Shibata, Y.

:: Circulation research, 巻 70, 番号 2, 01.01.1992, p. 370-381.

研究成果: ジャーナルへの寄稿記事

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abstract = "Bovine heart 67-kd protein (p67) was coisolated with calpactin I complex by cycles of Ca2+-dependent precipitation followed by solubilization with EGTA-containing buffer. Using affinity-purified anti-p67 antibody and anti-p36 (36-kd subunit of calpactin I) antibody, we examined the localization of the two proteins in secretory atrial myocytes and other endocrine tissues of adult rats. Immunofluorescence microscopy showed that p67 was expressed both in the atrial myocytes in situ and in cultured atrial myocytes in which we failed to detect p36 and that p67 appeared to be closely associated with the cell surface. We also found that p67 was colocalized with p36 in the thyroid follicle epithelium and zona reticularis of the adrenal gland. On the other hand, neither p67 nor p36 was detectable in pancreas islet cells. Immunoelectron microscopy revealed that p67 was localized at the sarcolemma in the atrial myocytes in situ. The p67, which was shown to be a globular molecule with a diameter of 18-25 nm by a low-angle rotary shadowing method, bound radioactive Ca2+ on a nitrocellulose membrane. The results suggest that Ca2+-binding proteins expressed in endocrine cells seem to vary from tissue to tissue and that p67 may function in Ca2+-mediated events at the plasma membrane of secretory atrial myocytes and some types of endocrine cells expressing this protein.",
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