Protein-retention expansion microscopy of cells and tissues labeled using standard fluorescent proteins and antibodies

Paul W. Tillberg, Fei Chen, Kiryl D. Piatkevich, Yongxin Zhao, C. C. Yu, Brian P. English, Linyi Gao, Anthony Martorell, Ho Jun Suk, Fumiaki Yoshida, Ellen M. Degennaro, Douglas H. Roossien, Guanyu Gong, Uthpala Seneviratne, Steven R. Tannenbaum, Robert Desimone, Dawen Cai, Edward S. Boyden

研究成果: Contribution to journalArticle査読

203 被引用数 (Scopus)

抄録

Expansion microscopy (ExM) enables imaging of preserved specimens with nanoscale precision on diffraction-limited instead of specialized super-resolution microscopes. ExM works by physically separating fluorescent probes after anchoring them to a swellable gel. The first ExM method did not result in the retention of native proteins in the gel and relied on custom-made reagents that are not widely available. Here we describe protein retention ExM (proExM), a variant of ExM in which proteins are anchored to the swellable gel, allowing the use of conventional fluorescently labeled antibodies and streptavidin, and fluorescent proteins. We validated and demonstrated the utility of proExM for multicolor super-resolution (∼1/470 nm) imaging of cells and mammalian tissues on conventional microscopes.

本文言語英語
ページ(範囲)987-992
ページ数6
ジャーナルNature Biotechnology
34
9
DOI
出版ステータス出版済み - 9 1 2016

All Science Journal Classification (ASJC) codes

  • バイオテクノロジー
  • バイオエンジニアリング
  • 応用微生物学とバイオテクノロジー
  • 分子医療
  • 生体医工学

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