Proteolytic cleavage of vitamin K-dependent bovine plasma protein S by α-thrombin and plasma serine protease

Takashi Morita, Jun Mizuguchi, Shun-Ichiro Kawabata, Sadaaki Iwanaga

研究成果: Contribution to journalArticle査読

2 被引用数 (Scopus)

抄録

It is known that protein S, a vitamin K-dependent plasma protein, isolated from a human source, gives a closely spaced doublet on sodium dodecyl sulfate-polyacryl amide gel electrophoresis after reduction and that this heterogeneity in molecular size results from a limited proteolysis of protein S mediated by α-thrombin in human species. We found here that a-thrombin also rapidly converted single-chain bovine protein S to a nicked form, which consisted of the NH2 segment containing γ-carboxyglutamic acid and the COOH-terminal large segment bridged by a distilfide linkage(s). These two segments were isolated and chemically characterized after S-alkylation of the nicked protein S. The results suggest that the α-thrombin-catalyzed hydrolysis of protein S probably occurs at a peptide linkage (Arg-Ser) located in the NH2 portion. The conversion of single-chain protein S to the nicked form was also mediated by plasma kallikrein and plasmin, in addition to α-chymotrypsin and trypsin. However, the α-thrombin-catalyzed conversion did not occur when calcium ions were added to the reaction mixture.

本文言語英語
ページ(範囲)561-568
ページ数8
ジャーナルJournal of biochemistry
99
2
DOI
出版ステータス出版済み - 1 1 1986

All Science Journal Classification (ASJC) codes

  • Biochemistry
  • Molecular Biology

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