Proteome analysis of silk gland proteins from the silkworm, Bombyx mori

Pingbo Zhang, Yoichi Aso, Kohji Yamamoto, Yutaka Banno, Yongqiang Wang, Kozo Tsuchida, Yutaka Kawaguchi, Hiroshi Fujii

研究成果: ジャーナルへの寄稿記事

67 引用 (Scopus)

抄録

The silk gland of Bombyx mori is an organ specialized for the synthesis and secretion of silk proteins. We report here the resolution of silk gland proteins by 2-DE and the identification of many of those proteins. This was accomplished by dissecting the glands into several sections, with each exhibiting more than 400 protein spots by 2-DE, of which 100 spots were excised and characterized by in-gel digestion followed by PMF. Ninety-three proteins were tentatively identified. These were then categorized into groups involved in silk protein secretion, transport, lipid metabolism, defense, etc. Western blotting of a 2-DE gel using an antibody of the carotenoid binding protein confirmed the presence of this protein in the silk gland. Proteins including fibroin L-chain and P25 were found as multiple isoforms, some of which contained differential amounts of phosphate residues as analyzed by on-probe dephosphorylation. The current analysis contributes to our understanding of proteins expressed by the silk gland not only of the model lepidopteran B. mori, but also to proteins from other silk-producing insects such as Philosamia cynthia ricini.

元の言語英語
ページ(範囲)2586-2599
ページ数14
ジャーナルProteomics
6
発行部数8
DOI
出版物ステータス出版済み - 4 1 2006

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Bombyx
Silk
Proteome
Proteins
Gels
Fibroins
Protein Transport
Carotenoids
Lipid Metabolism
Insects
Digestion
Carrier Proteins
Protein Isoforms
Western Blotting
Phosphates
Antibodies

All Science Journal Classification (ASJC) codes

  • Biochemistry
  • Molecular Biology

これを引用

Proteome analysis of silk gland proteins from the silkworm, Bombyx mori. / Zhang, Pingbo; Aso, Yoichi; Yamamoto, Kohji; Banno, Yutaka; Wang, Yongqiang; Tsuchida, Kozo; Kawaguchi, Yutaka; Fujii, Hiroshi.

:: Proteomics, 巻 6, 番号 8, 01.04.2006, p. 2586-2599.

研究成果: ジャーナルへの寄稿記事

Zhang, P, Aso, Y, Yamamoto, K, Banno, Y, Wang, Y, Tsuchida, K, Kawaguchi, Y & Fujii, H 2006, 'Proteome analysis of silk gland proteins from the silkworm, Bombyx mori', Proteomics, 巻. 6, 番号 8, pp. 2586-2599. https://doi.org/10.1002/pmic.200500348
Zhang, Pingbo ; Aso, Yoichi ; Yamamoto, Kohji ; Banno, Yutaka ; Wang, Yongqiang ; Tsuchida, Kozo ; Kawaguchi, Yutaka ; Fujii, Hiroshi. / Proteome analysis of silk gland proteins from the silkworm, Bombyx mori. :: Proteomics. 2006 ; 巻 6, 番号 8. pp. 2586-2599.
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