Proteomic analysis of in vivo 14-3-3 interactions in the yeast Saccharomyces cerevisiae

Kazue Kakiuchi, Yoshio Yamauchi, Masato Taoka, Maki Iwago, Tomoko Fujita, Takashi Ito, Si Young Song, Akira Sakai, Toshiaki Isobe, Tohru Ichimura

研究成果: ジャーナルへの寄稿記事

56 引用 (Scopus)

抄録

The yeast Saccharomyces cerevisiae produces two 14-3-3 proteins, Bmh1 and Bmh2, whose exact functions have remained unclear. Here, we performed a comprehensive proteomic analysis using multistep immunoaffinity purification and mass spectrometry and identified 271 yeast proteins that specifically bind to Bmh1 and -2 in a phosphorylation-dependent manner. The identified proteins have diverse biochemical functions and cellular roles, including cell signaling, metabolism, and cell cycle regulation. Importantly, there are a number of protein subsets that are involved in the regulation of yeast physiology through a variety of cell signaling pathways, including stress-induced transcription, cell division, and chitin synthesis at the cell wall. In fact, we found that a yeast mutant deficient in Bmh1 and -2 had defects in signal-dependent response of the MAPK (Hog1 and Mpk1) cascade and exhibited an abnormal accumulation of chitin at the bud neck. We propose that Bmh1 and -2 are common regulators of many cell signaling modules and pathways mediated by protein phosphorylation and regulate a variety of biological events by coordinately controlling the identified multiplex phosphoprotein components.

元の言語英語
ページ(範囲)7781-7792
ページ数12
ジャーナルBiochemistry
46
発行部数26
DOI
出版物ステータス出版済み - 7 3 2007

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Cell signaling
Proteomics
Yeast
Saccharomyces cerevisiae
Chitin
Yeasts
Phosphorylation
Cells
14-3-3 Proteins
Proteins
Fungal Proteins
Phosphoproteins
Cell Division
Cell Wall
Mass Spectrometry
Cell Cycle
Physiology
Transcription
Metabolism
Purification

All Science Journal Classification (ASJC) codes

  • Biochemistry

これを引用

Kakiuchi, K., Yamauchi, Y., Taoka, M., Iwago, M., Fujita, T., Ito, T., ... Ichimura, T. (2007). Proteomic analysis of in vivo 14-3-3 interactions in the yeast Saccharomyces cerevisiae. Biochemistry, 46(26), 7781-7792. https://doi.org/10.1021/bi700501t

Proteomic analysis of in vivo 14-3-3 interactions in the yeast Saccharomyces cerevisiae. / Kakiuchi, Kazue; Yamauchi, Yoshio; Taoka, Masato; Iwago, Maki; Fujita, Tomoko; Ito, Takashi; Song, Si Young; Sakai, Akira; Isobe, Toshiaki; Ichimura, Tohru.

:: Biochemistry, 巻 46, 番号 26, 03.07.2007, p. 7781-7792.

研究成果: ジャーナルへの寄稿記事

Kakiuchi, K, Yamauchi, Y, Taoka, M, Iwago, M, Fujita, T, Ito, T, Song, SY, Sakai, A, Isobe, T & Ichimura, T 2007, 'Proteomic analysis of in vivo 14-3-3 interactions in the yeast Saccharomyces cerevisiae', Biochemistry, 巻. 46, 番号 26, pp. 7781-7792. https://doi.org/10.1021/bi700501t
Kakiuchi K, Yamauchi Y, Taoka M, Iwago M, Fujita T, Ito T その他. Proteomic analysis of in vivo 14-3-3 interactions in the yeast Saccharomyces cerevisiae. Biochemistry. 2007 7 3;46(26):7781-7792. https://doi.org/10.1021/bi700501t
Kakiuchi, Kazue ; Yamauchi, Yoshio ; Taoka, Masato ; Iwago, Maki ; Fujita, Tomoko ; Ito, Takashi ; Song, Si Young ; Sakai, Akira ; Isobe, Toshiaki ; Ichimura, Tohru. / Proteomic analysis of in vivo 14-3-3 interactions in the yeast Saccharomyces cerevisiae. :: Biochemistry. 2007 ; 巻 46, 番号 26. pp. 7781-7792.
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AU - Ito, Takashi

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