Proteomic analysis of in vivo 14-3-3 interactions in the yeast Saccharomyces cerevisiae

Kazue Kakiuchi, Yoshio Yamauchi, Masato Taoka, Maki Iwago, Tomoko Fujita, Takashi Ito, Si Young Song, Akira Sakai, Toshiaki Isobe, Tohru Ichimura

研究成果: ジャーナルへの寄稿学術誌査読

65 被引用数 (Scopus)


The yeast Saccharomyces cerevisiae produces two 14-3-3 proteins, Bmh1 and Bmh2, whose exact functions have remained unclear. Here, we performed a comprehensive proteomic analysis using multistep immunoaffinity purification and mass spectrometry and identified 271 yeast proteins that specifically bind to Bmh1 and -2 in a phosphorylation-dependent manner. The identified proteins have diverse biochemical functions and cellular roles, including cell signaling, metabolism, and cell cycle regulation. Importantly, there are a number of protein subsets that are involved in the regulation of yeast physiology through a variety of cell signaling pathways, including stress-induced transcription, cell division, and chitin synthesis at the cell wall. In fact, we found that a yeast mutant deficient in Bmh1 and -2 had defects in signal-dependent response of the MAPK (Hog1 and Mpk1) cascade and exhibited an abnormal accumulation of chitin at the bud neck. We propose that Bmh1 and -2 are common regulators of many cell signaling modules and pathways mediated by protein phosphorylation and regulate a variety of biological events by coordinately controlling the identified multiplex phosphoprotein components.

出版ステータス出版済み - 7月 3 2007

!!!All Science Journal Classification (ASJC) codes

  • 生化学


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