Proteomic profiling of the silkworm skeletal muscle proteins during larval-pupal metamorphosis

Pingbo Zhang, Yoichi Aso, Hiroyuki Jikuya, Takahiro Kusakabe, Man Lee, Yutaka Kawaguchi, Kohji Yamamoto, Yutaka Banno, Hiroshi Fujii

研究成果: Contribution to journalArticle査読

21 被引用数 (Scopus)

抄録

The silkworm is a typical holometabolous insect going through drastic morphological changes upon metamorphosis from larvae to pupae. Comprehensive studies focusing on the changes help elucidate understanding of a biogenic mechanism. Here, we report the initial profile of the intersegmental muscle (ISM) proteins of the silkworm during larval-pupal metamorphosis. In total, 258 protein spots were resolved by two-dimensional gel electrophoresis (2-DE). Fifty-seven larval proteins were identified, where 3 proteins were exclusively detected in larval samples. Fifty-four other proteins were common in pupal samples. Of these, 12 proteins belonging to the contractile apparatus, metabolism, regulation, and signal transduction were altered in their contents during the metamorphosis from larvae to pupae. Three pupa-defective proteins were identified as isoforms of troponin I, followed by an immunoblotting validation. This data will be helpful in understanding the biochemistry of an insect ISM.

本文言語英語
ページ(範囲)2295-2303
ページ数9
ジャーナルJournal of Proteome Research
6
6
DOI
出版ステータス出版済み - 6 1 2007

All Science Journal Classification (ASJC) codes

  • 遺伝学
  • バイオテクノロジー
  • 生化学

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