Proteomic profiling of the silkworm skeletal muscle proteins during larval-pupal metamorphosis

Pingbo Zhang, Yoichi Aso, Hiroyuki Jikuya, Takahiro Kusakabe, Man Lee, Yutaka Kawaguchi, Kohji Yamamoto, Yutaka Banno, Hiroshi Fujii

研究成果: ジャーナルへの寄稿記事

21 引用 (Scopus)

抄録

The silkworm is a typical holometabolous insect going through drastic morphological changes upon metamorphosis from larvae to pupae. Comprehensive studies focusing on the changes help elucidate understanding of a biogenic mechanism. Here, we report the initial profile of the intersegmental muscle (ISM) proteins of the silkworm during larval-pupal metamorphosis. In total, 258 protein spots were resolved by two-dimensional gel electrophoresis (2-DE). Fifty-seven larval proteins were identified, where 3 proteins were exclusively detected in larval samples. Fifty-four other proteins were common in pupal samples. Of these, 12 proteins belonging to the contractile apparatus, metabolism, regulation, and signal transduction were altered in their contents during the metamorphosis from larvae to pupae. Three pupa-defective proteins were identified as isoforms of troponin I, followed by an immunoblotting validation. This data will be helpful in understanding the biochemistry of an insect ISM.

元の言語英語
ページ(範囲)2295-2303
ページ数9
ジャーナルJournal of Proteome Research
6
発行部数6
DOI
出版物ステータス出版済み - 6 1 2007

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Bombyx
Muscle Proteins
Proteomics
Skeletal Muscle
Pupa
Proteins
Larva
Insects
Signal transduction
Biochemistry
Troponin I
Electrophoresis, Gel, Two-Dimensional
Electrophoresis
Immunoblotting
Metabolism
Muscle
Signal Transduction
Protein Isoforms
Gels
Muscles

All Science Journal Classification (ASJC) codes

  • Genetics
  • Biotechnology
  • Biochemistry

これを引用

Proteomic profiling of the silkworm skeletal muscle proteins during larval-pupal metamorphosis. / Zhang, Pingbo; Aso, Yoichi; Jikuya, Hiroyuki; Kusakabe, Takahiro; Lee, Man; Kawaguchi, Yutaka; Yamamoto, Kohji; Banno, Yutaka; Fujii, Hiroshi.

:: Journal of Proteome Research, 巻 6, 番号 6, 01.06.2007, p. 2295-2303.

研究成果: ジャーナルへの寄稿記事

Zhang, Pingbo ; Aso, Yoichi ; Jikuya, Hiroyuki ; Kusakabe, Takahiro ; Lee, Man ; Kawaguchi, Yutaka ; Yamamoto, Kohji ; Banno, Yutaka ; Fujii, Hiroshi. / Proteomic profiling of the silkworm skeletal muscle proteins during larval-pupal metamorphosis. :: Journal of Proteome Research. 2007 ; 巻 6, 番号 6. pp. 2295-2303.
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AU - Lee, Man

AU - Kawaguchi, Yutaka

AU - Yamamoto, Kohji

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