抄録
The silkworm is a typical holometabolous insect going through drastic morphological changes upon metamorphosis from larvae to pupae. Comprehensive studies focusing on the changes help elucidate understanding of a biogenic mechanism. Here, we report the initial profile of the intersegmental muscle (ISM) proteins of the silkworm during larval-pupal metamorphosis. In total, 258 protein spots were resolved by two-dimensional gel electrophoresis (2-DE). Fifty-seven larval proteins were identified, where 3 proteins were exclusively detected in larval samples. Fifty-four other proteins were common in pupal samples. Of these, 12 proteins belonging to the contractile apparatus, metabolism, regulation, and signal transduction were altered in their contents during the metamorphosis from larvae to pupae. Three pupa-defective proteins were identified as isoforms of troponin I, followed by an immunoblotting validation. This data will be helpful in understanding the biochemistry of an insect ISM.
| 元の言語 | 英語 |
|---|---|
| ページ（範囲） | 2295-2303 |
| ページ数 | 9 |
| ジャーナル | Journal of Proteome Research |
| 巻 | 6 |
| 発行部数 | 6 |
| DOI | |
| 出版物ステータス | 出版済み - 6 1 2007 |
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All Science Journal Classification (ASJC) codes
- Genetics
- Biotechnology
- Biochemistry
これを引用
Proteomic profiling of the silkworm skeletal muscle proteins during larval-pupal metamorphosis. / Zhang, Pingbo; Aso, Yoichi; Jikuya, Hiroyuki; Kusakabe, Takahiro; Lee, Man; Kawaguchi, Yutaka; Yamamoto, Kohji; Banno, Yutaka; Fujii, Hiroshi.
：: Journal of Proteome Research, 巻 6, 番号 6, 01.06.2007, p. 2295-2303.研究成果: ジャーナルへの寄稿 › 記事
}
TY - JOUR
T1 - Proteomic profiling of the silkworm skeletal muscle proteins during larval-pupal metamorphosis
AU - Zhang, Pingbo
AU - Aso, Yoichi
AU - Jikuya, Hiroyuki
AU - Kusakabe, Takahiro
AU - Lee, Man
AU - Kawaguchi, Yutaka
AU - Yamamoto, Kohji
AU - Banno, Yutaka
AU - Fujii, Hiroshi
PY - 2007/6/1
Y1 - 2007/6/1
N2 - The silkworm is a typical holometabolous insect going through drastic morphological changes upon metamorphosis from larvae to pupae. Comprehensive studies focusing on the changes help elucidate understanding of a biogenic mechanism. Here, we report the initial profile of the intersegmental muscle (ISM) proteins of the silkworm during larval-pupal metamorphosis. In total, 258 protein spots were resolved by two-dimensional gel electrophoresis (2-DE). Fifty-seven larval proteins were identified, where 3 proteins were exclusively detected in larval samples. Fifty-four other proteins were common in pupal samples. Of these, 12 proteins belonging to the contractile apparatus, metabolism, regulation, and signal transduction were altered in their contents during the metamorphosis from larvae to pupae. Three pupa-defective proteins were identified as isoforms of troponin I, followed by an immunoblotting validation. This data will be helpful in understanding the biochemistry of an insect ISM.
AB - The silkworm is a typical holometabolous insect going through drastic morphological changes upon metamorphosis from larvae to pupae. Comprehensive studies focusing on the changes help elucidate understanding of a biogenic mechanism. Here, we report the initial profile of the intersegmental muscle (ISM) proteins of the silkworm during larval-pupal metamorphosis. In total, 258 protein spots were resolved by two-dimensional gel electrophoresis (2-DE). Fifty-seven larval proteins were identified, where 3 proteins were exclusively detected in larval samples. Fifty-four other proteins were common in pupal samples. Of these, 12 proteins belonging to the contractile apparatus, metabolism, regulation, and signal transduction were altered in their contents during the metamorphosis from larvae to pupae. Three pupa-defective proteins were identified as isoforms of troponin I, followed by an immunoblotting validation. This data will be helpful in understanding the biochemistry of an insect ISM.
UR - http://www.scopus.com/inward/record.url?scp=34250843824&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=34250843824&partnerID=8YFLogxK
U2 - 10.1021/pr070071y
DO - 10.1021/pr070071y
M3 - Article
C2 - 17497908
AN - SCOPUS:34250843824
VL - 6
SP - 2295
EP - 2303
JO - Journal of Proteome Research
JF - Journal of Proteome Research
SN - 1535-3893
IS - 6
ER -