Proteomic studies of isoforms of the P25 component of Bombyx mori fibroin

Pingbo Zhang, Kohji Yamamoto, Yoichi Aso, Yutaka Banno, Daisuke Sakano, Yongqiang Wang, Hiroshi Fujii

研究成果: ジャーナルへの寄稿記事

17 引用 (Scopus)

抄録

It is recognized that P25 is one of three polypeptide components of the fibroin synthesized in the larval silk gland (SG) of silkworm, having two glycosylated isoforms. In the present study, however, eight P25 isoforms were separated by proteomics, including two-dimensional gel electrophoresis of whole SG proteins, and were identified by the peptide mass fingerprinting method. Four of the eight isoforms were identified as Bombyx mandarina P25s, although the SG of Bombyx mori has never been considered to contain the P25 from B. mandarina. It is suggested that this diversity of P25 isoforms depends on phosphorylation modification in addition to glycosylation.

元の言語英語
ページ(範囲)2086-2093
ページ数8
ジャーナルBioscience, Biotechnology and Biochemistry
69
発行部数11
DOI
出版物ステータス出版済み - 12 8 2005

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Fibroins
Bombyx
Proteomics
Silk
Protein Isoforms
Glycosylation
Peptides
Phosphorylation
Peptide Mapping
Electrophoresis, Gel, Two-Dimensional
Electrophoresis
Gels
Proteins

All Science Journal Classification (ASJC) codes

  • Biotechnology
  • Analytical Chemistry
  • Biochemistry
  • Applied Microbiology and Biotechnology
  • Molecular Biology
  • Organic Chemistry

これを引用

Proteomic studies of isoforms of the P25 component of Bombyx mori fibroin. / Zhang, Pingbo; Yamamoto, Kohji; Aso, Yoichi; Banno, Yutaka; Sakano, Daisuke; Wang, Yongqiang; Fujii, Hiroshi.

:: Bioscience, Biotechnology and Biochemistry, 巻 69, 番号 11, 08.12.2005, p. 2086-2093.

研究成果: ジャーナルへの寄稿記事

Zhang, Pingbo ; Yamamoto, Kohji ; Aso, Yoichi ; Banno, Yutaka ; Sakano, Daisuke ; Wang, Yongqiang ; Fujii, Hiroshi. / Proteomic studies of isoforms of the P25 component of Bombyx mori fibroin. :: Bioscience, Biotechnology and Biochemistry. 2005 ; 巻 69, 番号 11. pp. 2086-2093.
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